The entomopathogenic fungi (EPF), as a biological control agent, can provide an alternative option for high-risk insecticides. Beauveria bassiana is one of the most potential EPF of pest control all over the world. The EPF isolate Y-F_ITS1 was isolated from Egyptian fauna on Beauveria-specific selective medium (BS medium). Molecular screening of the B. bassiana isolate, using PCR amplification with B. bassiana-specific primers and nucleotide sequence analysis of the internal transcribed spacer (ITS) region, confirmed the isolate as B. bassiana (accession no. [MK773644.1]). Efficacy of the isolate Y-F_ITS1 was examined against fourth instar larvae of Galleria mellonella L. as a model insect using concentrations from 104 to 107 spores ml−1 in a contact toxicity assay under laboratory conditions. The pathogenicity experiment showed that all the tested concentrations caused mortalities ranged from 75 to 98.33%, within 5 days posttreatment with cumulative mortalities, reached 100%, before 7 days with typical symptoms of infection and sporulation. Further investigations are needed to prove its efficacy against different economic pests as a credible candidate of integrated pest management (IPM) program.

中文翻译：

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隔离，土著的分子表征白僵菌分离物，使用ITS-5.8小号rDNA的区域和其对最大蜡蛾功效，大蜡螟L.（鳞翅目：螟蛾科）作为模型昆虫

昆虫病原真菌（EPF）作为生物防治剂，可以为高危杀虫剂提供替代选择。球孢白僵菌是全世界最有潜力的害虫防治EPF之一。EPF分离株Y-F_ITS1是在白僵菌特异性选择培养基（BS培养基）上从埃及动物中分离得到的。利用B. bassiana特异引物的PCR扩增和内部转录间隔区（ITS）区域的核苷酸序列分析，对B. bassiana分离株进行分子筛查，确认该分离株为B. bassiana（保藏号[MK773644.1]） 。在实验室条件下，在接触毒性试验中，使用104至107孢子ml-1的浓度，对分离的Y-F_ITS1的功效作为模型昆虫，对拱廊的第四龄幼虫进行了检测。致病性实验表明，所测试的所有致死浓度均在治疗后5天内以累积致死率在75至98.33％范围内，累积致死率达到100％，在7天内出现典型的感染和孢子形成症状。作为综合性有害生物管理（IPM）计划的可靠候选者，需要进一步研究以证明其对不同经济有害生物的功效。