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Continuous production of uniform chitosan beads as hemostatic dressings by a facile flow injection method.
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2020-07-15 , DOI: 10.1039/d0tb01462a Boxuan Li 1 , Juan Wang , Qin Gui , Hu Yang
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2020-07-15 , DOI: 10.1039/d0tb01462a Boxuan Li 1 , Juan Wang , Qin Gui , Hu Yang
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In this work, we developed a facile flow injection method to fabricate chitosan beads of uniform size in a continuous manner and assessed their properties as hemostatic dressings. Chitosan was dissolved into the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIM Ac) to form a solution, and then the chitosan/EMIM Ac solution was flow injected into ethanol to form beads of uniform size. The formed chitosan beads were obtained following the exchange of ethanol solvent with water and freeze-drying. The overall process is ecofriendly and scalable without acid/alkali treatment or the use of cross-linking agents. The morphology, swelling, and cytotoxicity of the chitosan beads were characterized. The blood coagulation and whole blood clotting kinetics of the chitosan beads were also studied. The chitosan beads have a significantly high swelling capacity. They can absorb 30 times as much water and 40 times as much PBS/0.9% NaCl solution as their dry mass. Cytotoxicity was not observed on NIH3T3 fibroblast cells after 24 h- or 48 h-incubation, when the concentration of the chitosan beads was 1.2 mg mL−1 or lower. Pig whole blood quickly lost its flowability in 10 min after the blood (1 mL) was incubated with 1 mg of chitosan beads. The whole blood clotting kinetics results showed that the chitosan beads efficiently caused the pig whole blood to clot with the absorbance of red blood cells (RBCs) dramatically reduced in 20 min and further reduced in 1 hour. The chitosan beads were shown to be biocompatible with excellent hemostatic properties. This work can apply a simple method to prepare chitosan beads for trauma hemostasis and broaden chitosan processing such as continuous manufacturing and 3D printing.
中文翻译：
通过简便的流动注射法连续生产均匀的壳聚糖珠作为止血敷料。
在这项工作中，我们开发了一种简便的流动注射方法，以连续方式制造大小均一的壳聚糖珠，并评估了其作为止血敷料的性能。将脱乙酰壳多糖溶解在离子液体乙酸1-乙基-3-甲基咪唑鎓盐（EMIM Ac）中形成溶液，然后将脱乙酰壳多糖/ EMIM Ac溶液流注入乙醇中以形成大小均一的珠。将乙醇溶剂与水交换并冷冻干燥后，获得形成的壳聚糖珠。整个过程是环保的，无需酸/碱处理或使用交联剂即可扩展。表征了壳聚糖珠的形态，溶胀和细胞毒性。还研究了壳聚糖珠的凝血和全血凝固动力学。壳聚糖珠粒具有显着高的溶胀能力。它们吸收的水分和干燥质量是其PBS / 0.9％NaCl溶液的30倍和40倍。当壳聚糖珠的浓度为1.2 mg mL时，在孵育24 h或48 h后未观察到NIH3T3成纤维细胞的细胞毒性-1或更低。将猪血（1 mL）与1 mg壳聚糖珠一起孵育后10分钟，猪全血迅速失去流动性。全血凝固动力学结果表明，壳聚糖珠有效地使猪的全血凝结，其中红细胞（RBC）的吸光度在20分钟内急剧下降，并在1小时内进一步下降。壳聚糖珠粒具有良好的止血性能，具有生物相容性。这项工作可以应用一种简单的方法来制备用于创伤止血的壳聚糖珠，并扩大壳聚糖的加工范围，例如连续生产和3D打印。
更新日期：2020-09-16
中文翻译：
通过简便的流动注射法连续生产均匀的壳聚糖珠作为止血敷料。
在这项工作中，我们开发了一种简便的流动注射方法，以连续方式制造大小均一的壳聚糖珠，并评估了其作为止血敷料的性能。将脱乙酰壳多糖溶解在离子液体乙酸1-乙基-3-甲基咪唑鎓盐（EMIM Ac）中形成溶液，然后将脱乙酰壳多糖/ EMIM Ac溶液流注入乙醇中以形成大小均一的珠。将乙醇溶剂与水交换并冷冻干燥后，获得形成的壳聚糖珠。整个过程是环保的，无需酸/碱处理或使用交联剂即可扩展。表征了壳聚糖珠的形态，溶胀和细胞毒性。还研究了壳聚糖珠的凝血和全血凝固动力学。壳聚糖珠粒具有显着高的溶胀能力。它们吸收的水分和干燥质量是其PBS / 0.9％NaCl溶液的30倍和40倍。当壳聚糖珠的浓度为1.2 mg mL时，在孵育24 h或48 h后未观察到NIH3T3成纤维细胞的细胞毒性-1或更低。将猪血（1 mL）与1 mg壳聚糖珠一起孵育后10分钟，猪全血迅速失去流动性。全血凝固动力学结果表明，壳聚糖珠有效地使猪的全血凝结，其中红细胞（RBC）的吸光度在20分钟内急剧下降，并在1小时内进一步下降。壳聚糖珠粒具有良好的止血性能，具有生物相容性。这项工作可以应用一种简单的方法来制备用于创伤止血的壳聚糖珠，并扩大壳聚糖的加工范围，例如连续生产和3D打印。
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