Atypical porcine pestivirus (APPV) had been detected in many countries. However, to date, a commercial detection kit is not available because of a lack of specific monoclonal antibodies (mAbs) to APPV. We generated 7 mAbs targeting the NS3 protein of APPV. Isotyping results indicated that all of these mAbs are IgG1 with a kappa light chain. We analyzed the epitopes recognized by mAbs 2B6, 6G11, 8D1, 8D3, and 8F12, which recognized the same linear epitope (GRIKSAYSDE); the 6H3 and 7E10 mAbs recognized 2 different conformational epitopes. Applications of these antibodies were verified by ELISA, western blot, indirect immunofluorescence assay, and flow cytometry. The antibodies were functionally workable for these immunoassays except for 8F12, which could not be used in flow cytometry.

在许多国家已经检测到非典型猪瘟病毒（APPV）。但是，由于缺少针对APPV的特异性单克隆抗体（mAb），到目前为止，尚无商用检测试剂盒。我们产生了针对APPV NS3蛋白的7种单克隆抗体。同位素分型结果表明，所有这些mAb均为具有κ轻链的IgG1。我们分析了mAbs 2B6、6G11、8D1、8D3和8F12识别的表位，这些表位识别相同的线性表位（GRIKSAYSDE）；6H3和7E10单克隆抗体识别2种不同的构象表位。这些抗体的应用已通过ELISA，western印迹，间接免疫荧光测定和流式细胞仪进行了验证。除了8F12不能用于流式细胞术外，这些抗体在功能上可用于这些免疫分析。