γ-Hydroxybutyrate (GHB) is a neurotransmitter, which exhibits a strong central nervous system depressant effect. The abuse of GHB or its precursor substances (γ-butyrolactone (GBL) and 1,4-butanediol (1,4-BD)) may cause serious problems. This study developed a fast and effective UHPLC-MS/MS method for the simultaneous quantification of GHB, GBL, and 1,4-BD in four popular beverages, including carbonated drinks, tea, apple cider vinegar, and coffee. The established method overcomes the influence of the in-source collision-induced dissociation of unstable compounds during quantification. The limits of detection were 0.2 μg/mL for GBL and 0.5 μg/mL for GHB and 1,4-BD with excellent linearity in the range of 0.2–50 μg/mL. The recoveries of the three compounds at three spiked levels (2.5, 5.0, and 10.0 μg/mL) in the four kinds of beverages studied were between 90 and 110%, while the relative standard deviations (RSDs) were all <10%. The matrix effect was negligible using this simple and appropriate preprocessed procedure. The method established in this study can quickly and reliably detect the GHB content and its analogues in beverages.

中文翻译：

---

同时定量四种饮料中的γ-羟基丁酸，γ-丁内酯和1,4-丁二醇。

γ-羟基丁酸酯（GHB）是一种神经递质，具有很强的中枢神经系统抑制作用。滥用GHB或其前体物质（γ-丁内酯（GBL）和1,4-丁二醇（1,4-BD））可能会导致严重问题。这项研究开发了一种快速高效的UHPLC-MS / MS方法，用于同时定量四种碳酸饮料（包括碳酸饮料，茶，苹果酒醋和咖啡）中的GHB，GBL和1,4-BD。所建立的方法克服了在定量过程中源内碰撞引起的不稳定化合物解离的影响。检测限为0.2  μ克/ mL的GBL和0.5  μ克/毫升为GHB和1,4-BD与0.2-50的范围内优异的线性 μ克/毫升。三种化合物中的添加水平下的回收率（2.5，5.0和10.0  μ克/毫升）的四种类的饮料研究分别为90和110％之间，而相对标准偏差（RSD）为所有的<10％。使用这种简单而适当的预处理程序，基质效应可以忽略不计。在这项研究中建立的方法可以快速，可靠地检测饮料中GHB含量及其类似物。