Genetically encoded logic gates, especially inverters NOT gates are the building blocks for designing circuits, engineering biosensors or decision-making devices in synthetic biology. However, the repertoire of inverters readily available for different species is rather limited. In this work, a large whole of NOT gates that was shown to function previously in a specific strain of Escherichia coli, was recreated as broad host range (BHR) collection of constructs assembled in low, medium and high copy number plasmid backbones of the SEVA (Standard European Vector Architecture) collection. The input/output function of each of the gates was characterized and parameterized in the environmental bacterium and metabolic engineering chassis Pseudomonas putida. Comparisons of the resulting fluorescence cytometry data with those published for the same gates in Escherichia coli provided useful hints on the portability of the corresponding gates. The hereby described BHR inverter package (20 different versions of 12 distinct gates) thus becomes a toolbox of choice for designing genetic circuitries in a variety of Gram-negative species other than E. coli.

中文翻译：

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用于革兰氏阴性细菌遗传电路设计的标准化宽主机范围逆变器套件

遗传编码逻辑门，尤其是逆变器非门是合成生物学中设计电路，工程生物传感器或决策装置的基础。然而，对于不同种类容易获得的逆变器的种类相当有限。在这项工作中，重新创建了一大批在特定大肠杆菌中已发挥功能的非门，作为在SEVA的低，中和高拷贝数质粒主链中组装的构建体的广泛宿主范围（BHR）集合（标准欧洲矢量体系结构）集合。每个门的输入/输出功能在环境细菌和代谢工程底盘假单胞菌中进行了表征和参数化。将所得的荧光细胞计数数据与大肠杆菌中相同门的数据进行比较，可为相应门的便携性提供有用的提示。因此，此处描述的BHR逆变器套件（12个不同门的20个不同版本）因此成为设计除大肠杆菌以外的各种革兰氏阴性物种中遗传电路的首选工具箱。