Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
High-Aspect-Ratio SU-8-Based Optofluidic Device for Ammonia Detection in Cell Culture Media.
ACS Sensors ( IF 8.2 ) Pub Date : 2020-07-15 , DOI: 10.1021/acssensors.0c00821 Esma Dervisevic 1 , Nicolas H Voelcker 2, 3, 4 , Gail Risbridger 5, 6, 7 , Kellie L Tuck 8 , Victor J Cadarso 1, 3
ACS Sensors ( IF 8.2 ) Pub Date : 2020-07-15 , DOI: 10.1021/acssensors.0c00821 Esma Dervisevic 1 , Nicolas H Voelcker 2, 3, 4 , Gail Risbridger 5, 6, 7 , Kellie L Tuck 8 , Victor J Cadarso 1, 3
Affiliation
|
Miniaturization of sensing technology has led to the development of multifunctional micro total analysis systems (μTAS) that benefit from microfluidic technology. Optical sensing is one of the most commonly used sensing approaches integrated into μTAS devices and features high sensitivity and low detection limits. Different materials have been used for the fabrication of μTAS devices, each having their advantages and disadvantages. Herein, a high-aspect-ratio optofluidic waveguide fabricated from SU-8 is presented for the first time. The suitable optical properties and chemical inertness of SU-8 provide a durable device made by a flexible and cost-efficient fabrication process. The optofluidic device was used for colorimetric ammonia (NH3) sensing with a dynamic range of 3–70 μM, a detection limit of 2.5 μM, a response time of 8 min, and close to 10 times better analytical performance compared to using a standard microplate reader. The μTAS device was capable of monitoring NH3 accumulating in the cell culture media of prostatic epithelial cell (BPH-1) culture.
中文翻译：
基于高纵横比SU-8的细胞培养基中氨检测的光流装置。
传感技术的小型化已导致受益于微流体技术的多功能微量总分析系统（μTAS）的发展。光学传感是集成到μTAS设备中的最常用传感方法之一，具有高灵敏度和低检测限的特点。μTAS器件的制造已使用了不同的材料，每种材料都有其优点和缺点。本文中，首次提出了由SU-8制成的高纵横比的光导波导。SU-8的合适的光学性能和化学惰性提供了一种通过灵活且经济高效的制造工艺制造的耐用设备。光流体装置用于比色氨气（NH 3）的检测动态范围为3–70μM，检测极限为2.5μM，响应时间为8分钟，与使用标准酶标仪相比，分析性能提高了近10倍。μTAS设备能够监测在前列腺上皮细胞（BPH-1）培养的细胞培养基中积累的NH 3。
更新日期：2020-08-28
中文翻译：
基于高纵横比SU-8的细胞培养基中氨检测的光流装置。
传感技术的小型化已导致受益于微流体技术的多功能微量总分析系统（μTAS）的发展。光学传感是集成到μTAS设备中的最常用传感方法之一，具有高灵敏度和低检测限的特点。μTAS器件的制造已使用了不同的材料，每种材料都有其优点和缺点。本文中，首次提出了由SU-8制成的高纵横比的光导波导。SU-8的合适的光学性能和化学惰性提供了一种通过灵活且经济高效的制造工艺制造的耐用设备。光流体装置用于比色氨气（NH 3）的检测动态范围为3–70μM，检测极限为2.5μM，响应时间为8分钟，与使用标准酶标仪相比，分析性能提高了近10倍。μTAS设备能够监测在前列腺上皮细胞（BPH-1）培养的细胞培养基中积累的NH 3。
京公网安备 11010802027423号