Abstract

Natural antiparasitic agents are used as a strategy for advances in the treatment of neglected tropical diseases (NTDs), such as giardiasis. The present paper identified the compounds produced by Ganoderma lipsiense mycelium grown in submerged fermentation and investigated the antigiardial activity potential of extracts in vitro. G. lipsiense mycelium crude extract (CE) was obtained by the maceration process and after purification in column, thirty compounds were identified by gas chromatography - mass spectrometry (GC-MS) in hexane sub-fraction (HSF). To determine the antigiardial potential, different parts of the purified CE have been evaluated, where hexane (HEXf) and dichloromethane (DCMf) fractions showed in vitro reduction levels higher than 90% of the Giardia duodenalis trophozoites at 100 μg mL⁻¹. This antigiardial activity was investigated aiming to explore the possibility of G. lipsiense mycelium extract as a novel antigiardial compound to the treatment of human giardiasis.

摘要

天然抗寄生虫药被用作治疗被忽视的热带病 (NTD)（如贾第虫病）的一种策略。本文鉴定了在深层发酵中生长的灵芝菌丝体产生的化合物，并研究了提取物的体外抗贾第虫活性潜力。G.lipsiense菌丝体粗提物( CE )通过浸渍工艺获得，经柱纯化后，用气相色谱-质谱(GC-MS)在己烷亚馏分( HSF )中鉴定出30种化合物。为了确定抗贾第虫的潜力，对纯化CE 的不同部分进行了评估，其中己烷（HEXf) 和二氯甲烷 ( DCMf ) 级分在 100 μg mL ^{-1 时}显示出高于 90% 的十二指肠贾第鞭毛虫滋养体的体外减少水平。研究这种抗贾第虫活性的目的是探索G.lipsiense菌丝体提取物作为一种新型抗贾第虫化合物治疗人类贾第虫病的可能性。