Development of a rapid FLISA detection of Salmonella spp. based on CdTe/ZnS quantum dots,Journal of Food Safety

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Development of a rapid FLISA detection of Salmonella spp. based on CdTe/ZnS quantum dots
Journal of Food Safety ( IF 1.9 ) Pub Date : 2020-07-15 , DOI: 10.1111/jfs.12830
Xiaobao Qi ₁ , Huanhuan Xu ₂ , Wukang Liu ₁ , Ailing Guo _{1,

2}

Affiliation

Salmonella pathogen is old and difficult to control in food safety. The study was aimed to develop a sensitive and rapid CdTe/ZnS quantum dots (QDs) mediated fluorescence‐linked immunosorbent assay (FLISA) of Salmonella spp. The optical properties and structure of the CdTe/ZnS QDs were characterized by UV–Vis absorption spectroscopy and transmission electron microscopy. The emission peak wavelength is 575 nm. The CdTe/ZnS QDs‐labeled secondary antibody (goat anti‐rabbit IgG) was formed by biotin‐avidin system. The detection limits of S. Enteritidis and artificial simulated samples are 1.0 × 10³ CFU/ml and 1.78 × 10³ CFU/ml, respectively. The study of FLISA stability shows that the antibody with QDs had very good activity when it was stored in the dark at 4°C for at least 35 days. The FLISA provides a new and easy‐operated method for the rapid detection of Salmonella spp. and has broad application in food safety.

中文翻译：

沙门氏菌快速FLISA检测的开发。基于CdTe / ZnS量子点的

沙门氏菌病原体较旧，食品安全性难以控制。该研究旨在开发一种灵敏，快速的CdTe / ZnS量子点（QDs）介导的沙门氏菌属的荧光连锁免疫吸附测定（FLISA）。CdTe / ZnS量子点的光学性质和结构通过紫外可见吸收光谱和透射电子显微镜表征。发射峰波长为575 nm。CdTe / ZnS QDs标记的二抗（山羊抗兔IgG）是由生物素-亲和素系统形成的。的检测限S.肠炎和人工模拟的样品是1.0×10 ³ CFU / ml和1.78×10 ³CFU / ml。对FLISA稳定性的研究表明，带有QD的抗体在4°C的黑暗环境中保存至少35天时具有很好的活性。FLISA为快速检测沙门氏菌提供了一种新的且易于操作的方法。并在食品安全中有广泛的应用。

更新日期：2020-07-15

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