This study identifies the main changes in protein expression in human breast tumors compared to normal breast tissue. Malignant tumors (32) and normal breast tissue samples (23), from formaldehyde‐fixed, paraffin‐embedded specimens are subjected to discovery proteomics using liquid chromatography/tandem mass spectrometry, with spectral counts for quantitation. The dataset contains 1406 proteins. Differential expression is measured using a method that takes advantage of estimates of the percentage of tumor on a slide. This analysis shows that the major classes of proteins over‐expressed by tumors are RNA‐binding, heat shock and DNA repair proteins. RNA‐binding proteins, including heterogeneous nuclear ribonucleoproteins (HNRNPs), SR splice factors (SRSF) and elongation factors form the largest group. Comparison with results from another study demonstrates that the RNA‐binding proteins are associated specifically with malignant transformation, rather than with cell proliferation. HNRNP and SRSF proteins help define splice sites in normal cells. Their over‐expression may dysregulate splicing, which in turn has the potential to promote malignant transformation.

中文翻译：

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与正常组织相比，乳腺肿瘤中 RNA 加工、热激和 DNA 修复蛋白的过度表达。


这项研究确定了与正常乳腺组织相比，人类乳腺肿瘤中蛋白质表达的主要变化。使用液相色谱/串联质谱法对来自甲醛固定、石蜡包埋的标本的恶性肿瘤 (32) 和正常乳腺组织样本 (23) 进行发现蛋白质组学分析，并通过光谱计数进行定量。该数据集包含 1406 个蛋白质。使用利用载玻片上肿瘤百分比估计的方法来测量差异表达。该分析表明，肿瘤过度表达的主要蛋白质类别是 RNA 结合蛋白、热休克蛋白和 DNA 修复蛋白。 RNA 结合蛋白，包括异质核核糖核蛋白 (HNRNP)、SR 剪接因子 (SRSF) 和延伸因子构成了最大的一组。与另一项研究结果的比较表明，RNA 结合蛋白与恶性转化特异性相关，而不是与细胞增殖相关。 HNRNP 和 SRSF 蛋白有助于定义正常细胞中的剪接位点。它们的过度表达可能会导致剪接失调，进而有可能促进恶性转化。