Environmentally friendly green approach for the fabrication of silver oxide nanoparticles: Characterization and diverse biomedical applications.,Microscopy Research and Technique

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Environmentally friendly green approach for the fabrication of silver oxide nanoparticles: Characterization and diverse biomedical applications.
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2020-07-14 , DOI: 10.1002/jemt.23522
Banzeer Ahsan Abbasi ₁ , Javed Iqbal ₁ , Jamal Abdul Nasir ₂ , Syeda Anber Zahra ₁ , Amir Shahbaz ₁ , Siraj Uddin ₁ , Safia Hameed ₃ , Farhat Gul ₁ , Sobia Kanwal ₄ , Tariq Mahmood ₁

Affiliation

In the present study, green silver nanoparticles (Ag₂ONPs) were prepared from aqueous and ethanolic leaves extract of Rhamnus virgata in a facile, green, cost‐effective, and eco‐friendly way. The color changes from light brown to brownish black determined the synthesis of Ag₂ONPs_(Aq) and Ag₂ONPs_(Et). The phytofabrication of Ag₂ONPs was confirmed using various spectroscopic and microscopic techniques: energy‐dispersive X‐ray spectroscopy, dynamic light scattering, ultraviolet–visible spectroscopy, Fourier‐transform infrared, X‐ray powder diffraction, Raman, scanning electron microscopy, and transmission electron microscopy. Detailed in vitro biological activities determined significant biopotentials for Ag₂ONPs. The Ag₂ONPs_(Aq) and Ag₂ONPs_(Et) were investigated for anticancer potential against HUH‐7 (IC₅₀: 9.075 μg/ml for Ag₂O_(Aq) and 25.66 μg/ml for Ag₂O_(Et)) and HepG2 (IC₅₀: 25.18 μg/ml for Ag₂O_(Aq) and IC₅₀: 27.74 μg/ml for Ag₂O_(Aq)) cell lines. Concentration‐dependent cytotoxicity was performed against brine‐shrimps (IC₅₀: 36.04 μg/ml for Ag₂O_(Aq) and 28.82 μg/ml for Ag₂O_(Et)) and Leishmanial parasite (amastigotes and promastigotes). Disc‐diffusion method revealed significant antimicrobial activities. In addition, significant enzyme inhibitory activity and antiradical potentials were studied. The hemocompatible nature of Ag₂ONPs_(Aq) and Ag₂ONPs_(Et) was revealed using biocompatibility tests. In conclusion, the green Ag₂ONPs_(Aq) and Ag₂ONPs_(Et) are nontoxic and biocompatible and has shown significant biological activities. We further encourage in vivo studies to ensure biosafety and biocompatibility, so that they can be effectively utilized in nano‐pharmaceutical industries.

中文翻译：

用于制造氧化银纳米颗粒的环保绿色方法：表征和多样化的生物医学应用。

在本研究中，绿色银纳米粒子 (Ag ₂ ONPs) 是由鼠李的水和乙醇叶提取物以一种简便、绿色、具有成本效益和生态友好的方式制备的。从浅棕色到棕黑色的颜色变化决定了 Ag ₂ ONPs _(Aq)和 Ag ₂ ONPs _(Et)的合成。Ag ₂的植物加工使用各种光谱和显微技术证实了 ONPs：能量色散 X 射线光谱、动态光散射、紫外-可见光谱、傅里叶变换红外、X 射线粉末衍射、拉曼、扫描电子显微镜和透射电子显微镜。详细的体外生物活性决定了 Ag ₂ ONPs 的重要生物电势。研究了 Ag ₂ ONPs _(Aq)和 Ag ₂ ONPs _(Et)对 HUH-7 的抗癌潜力（IC ₅₀：Ag ₂ O _(Aq)为 9.075 μg/ml，Ag ₂ O _(Et)为 25.66 μg/ml _） ) 和 HepG2 (IC ₅₀：Ag ₂ O _(Aq)为 25.18 μg/ml ，IC ₅₀：Ag ₂ O _(Aq) ) 细胞系为 27.74 μg/ml 。对盐水虾（IC ₅₀：Ag ₂ O _(Aq)为 36.04 μg /ml，Ag ₂ O _(Et)为 28.82 μg/ml ）和利什曼原虫寄生虫（无鞭毛体和前鞭毛体）进行浓度依赖性细胞毒性。圆盘扩散法显示出显着的抗菌活性。此外，还研究了显着的酶抑制活性和抗自由基电位。Ag ₂ ONPs _(Aq)和 Ag ₂ ONPs _(Et)的血液相容性使用生物相容性测试揭示。总之，绿色的 Ag ₂ ONPs _(Aq)和 Ag ₂ ONPs _(Et)是无毒和生物相容性的，并已显示出显着的生物活性。我们进一步鼓励体内研究以确保生物安全性和生物相容性，以便它们能够有效地用于纳米制药行业。

更新日期：2020-07-14

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