Butein, a flavonoid found in annatto seeds and lacquer trees, may be used for many health benefits, including the prevention of obesity. However, its anti‐obesity effects are not completely understood; in particular, the effects of butein on the regulation of lipid metabolism have not been explained. Thus, the goal of the current study was to determine the effects of butein on lipid metabolism in Caenorhabditis elegans, which is a multi‐organ nematode used as an animal model in obesity research. Butein at 70 μM reduced triglyceride content by 27% compared to the control without altering food intake and energy expenditure. The reduced triglyceride content by butein was associated with the downregulation of sbp‐1, fasn‐1, and fat‐7, the lipogenesis‐related homologs of sterol regulatory element‐binding proteins, fatty acid synthase and stearoyl‐CoA desaturase, respectively. Furthermore, fat‐7 and skn‐1, a homolog of nuclear respiratory factors, were identified as genetic requirements for butein's effects on triglyceride content in C. elegans. The effects of butein on sbp‐1 and fasn‐1 were dependent on skn‐1, but the downregulation of fat‐7 was independent of skn‐1. These results suggest that the inhibitory effects of butein on lipogenesis are via SKN‐1‐ and FAT‐7‐dependent pathways in C. elegans.

中文翻译：

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Butein 抑制秀丽隐杆线虫的脂肪生成。

Butein 是一种在胭脂树种子和漆树中发现的类黄酮，可用于许多健康益处，包括预防肥胖。然而，它的抗肥胖作用尚不完全清楚。尤其是黄豆素对脂质代谢调节的作用尚未得到解释。因此，本研究的目标是确定布丁素对秀丽隐杆线虫脂质代谢的影响，秀丽隐杆线虫是一种多器官线虫，在肥胖研究中用作动物模型。与对照相比，70 μM 的 Butein 将甘油三酯含量降低了 27%，而不会改变食物摄入和能量消耗。Butein 降低的甘油三酯含量与sbp-1、fasn-1和fat-7的下调有关，分别为甾醇调节元件结合蛋白、脂肪酸合酶和硬脂酰辅酶A去饱和酶的脂肪生成相关同源物。此外，核呼吸因子的同系物fat-7和skn-1被确定为布丁素对秀丽隐杆线虫中甘油三酯含量影响的遗传要求。Butein 对sbp-1和fasn-1 的影响依赖于skn-1，但fat-7的下调与skn-1无关。这些结果表明，布丁对脂肪生成的抑制作用是通过秀丽隐杆线虫中的SKN-1 和 FAT-7 依赖性途径实现的。