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Modulation of octopaminergic and cholinergic pathways induced by Caatinga tree Manilkara rufula chemical compounds in Nauphoeta cinerea cockroaches
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.pestbp.2020.104651 Bruna Trindade Borges 1 , Patrícia de Brum Vieira 2 , Allan P Leal 3 , Etiely Karnopp 4 , Bárbara A B Ogata 4 , Maria Eduarda Rosa 1 , Yuri Correia Barreto 4 , Raquel Soares Oliveira 1 , Cháriston André Dal Belo 5 , Lúcia Vinadé 1
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.pestbp.2020.104651 Bruna Trindade Borges 1 , Patrícia de Brum Vieira 2 , Allan P Leal 3 , Etiely Karnopp 4 , Bárbara A B Ogata 4 , Maria Eduarda Rosa 1 , Yuri Correia Barreto 4 , Raquel Soares Oliveira 1 , Cháriston André Dal Belo 5 , Lúcia Vinadé 1
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The entomotoxic potential of Manilkara rufula crude extract (CEMR) and its aqueous (AFMR) and methanolic (MFMR) fractions were evaluated against Nauphoeta cinerea cockroaches. The results point out to a direct modulation of octopaminergic and cholinergic pathways in insect nervous system. CEMR induced an anti-acetylcholinesterase (AChE) effect in cockroach brain homogenates. CEMR significantly decreased the cockroach heart rate in semi-isolated heart preparations. CEMR also caused a broad disturbance in the insect behavior by reducing the exploratory activity. The decreased antennae and leg grooming activities, by different doses of CEMR, mimicked those of phentolamine activity, a selective octopaminergic receptor antagonist. The lethargy induced by CEMR was accompanied by neuromuscular failure and by a decrease of sensilla spontaneous neural compound action potentials (SNCAP) firing in in vivo and ex vivo cockroach muscle-nerve preparations, respectively. AFMR was more effective in promoting neuromuscular paralysis than its methanolic counterpart, in the same dose. These data validate the entomotoxic activity of M. rufula. The phentolamine-like modulation induced in cockroaches is the result of a potential direct inhibition of octopaminergic receptors, combined to an anti-AChE activity. In addition, the modulation of CEMR on octopaminergic and cholinergic pathways is probably the result of a synergism between AFMR and MFMR chemical compounds. Further phytochemical investigation followed by a bio-guiding protocol will improve the molecular aspects of M. rufula pharmacology and toxicology to insects.
中文翻译：
在 Nauphoeta cinerea 蟑螂中由 Caatinga 树 Manilkara rufula 化合物诱导的章鱼胺能和胆碱能通路的调节
评估了 Manilkara rufula 粗提取物 (CEMR) 及其水溶液 (AFMR) 和甲醇 (MFMR) 馏分对 Nauphoeta cinerea 蟑螂的昆虫毒性潜力。结果指出昆虫神经系统中章鱼胺能和胆碱能通路的直接调节。CEMR 在蟑螂脑匀浆中诱导抗乙酰胆碱酯酶 (AChE) 作用。CEMR 显着降低了半隔离心脏制剂中的蟑螂心率。CEMR 还通过减少探索活动对昆虫行为造成广泛干扰。不同剂量的 CEMR 降低的触角和腿部修饰活动与酚妥拉明（一种选择性章鱼胺能受体拮抗剂）的活性相似。CEMR 诱导的嗜睡伴随着神经肌肉衰竭和感器自发性神经复合动作电位 (SNCAP) 在体内和体外蟑螂肌肉-神经制剂中的降低。在相同剂量下，AFMR 在促进神经肌肉麻痹方面比其甲醇对应物更有效。这些数据验证了 M. rufula 的昆虫毒性活性。在蟑螂中诱导的酚妥拉明样调节是潜在的直接抑制章鱼胺能受体的结果，结合抗乙酰胆碱酯酶活性。此外，CEMR 对章鱼胺能和胆碱能通路的调节可能是 AFMR 和 MFMR 化合物之间协同作用的结果。进一步的植物化学研究和生物指导协议将改善 M. 的分子方面。
更新日期：2020-10-01
中文翻译：
在 Nauphoeta cinerea 蟑螂中由 Caatinga 树 Manilkara rufula 化合物诱导的章鱼胺能和胆碱能通路的调节
评估了 Manilkara rufula 粗提取物 (CEMR) 及其水溶液 (AFMR) 和甲醇 (MFMR) 馏分对 Nauphoeta cinerea 蟑螂的昆虫毒性潜力。结果指出昆虫神经系统中章鱼胺能和胆碱能通路的直接调节。CEMR 在蟑螂脑匀浆中诱导抗乙酰胆碱酯酶 (AChE) 作用。CEMR 显着降低了半隔离心脏制剂中的蟑螂心率。CEMR 还通过减少探索活动对昆虫行为造成广泛干扰。不同剂量的 CEMR 降低的触角和腿部修饰活动与酚妥拉明（一种选择性章鱼胺能受体拮抗剂）的活性相似。CEMR 诱导的嗜睡伴随着神经肌肉衰竭和感器自发性神经复合动作电位 (SNCAP) 在体内和体外蟑螂肌肉-神经制剂中的降低。在相同剂量下，AFMR 在促进神经肌肉麻痹方面比其甲醇对应物更有效。这些数据验证了 M. rufula 的昆虫毒性活性。在蟑螂中诱导的酚妥拉明样调节是潜在的直接抑制章鱼胺能受体的结果，结合抗乙酰胆碱酯酶活性。此外，CEMR 对章鱼胺能和胆碱能通路的调节可能是 AFMR 和 MFMR 化合物之间协同作用的结果。进一步的植物化学研究和生物指导协议将改善 M. 的分子方面。
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