Tagitinin A from Tithonia diversifolia provides resistance to tomato spotted wilt orthotospovirus by inducing systemic resistance,Pesticide Biochemistry and Physiology

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Tagitinin A from Tithonia diversifolia provides resistance to tomato spotted wilt orthotospovirus by inducing systemic resistance
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.pestbp.2020.104654
Lihua Zhao ₁ , Zhonghui Hu ₂ , Shunlin Li ₂ , Lizhen Zhang ₁ , Ping Yu ₁ , Jie Zhang ₁ , Xue Zheng ₁ , Siddiqur Rahman ₃ , Zhongkai Zhang ₁

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Tomato spotted wilt orthotospovirus (TSWV) causes devastating losses to agronomic and ornamental crops worldwide. Currently, there is no effective strategy to control this disease. Use of biotic inducers to enhance plant resistance to viruses maybe an effective approach. Our previous study indicated that Tagitinin A (Tag A) has a high curative and protective effect against TSWV. However, the underlying molecular mechanism of Tag A-mediated antiviral activity remains unknown. In this study, Tag A reduced the expression of the NSs, NSm genes was very low in untreated leaves following TSWV infection. In addition, the expression of all TSWV genes in the inoculated and systemic leaves was inhibited in the protective assay, and with an inhibition rate of more than 85% in systemic leaves. Tag A increased phenylalanine ammonia-lyase (PAL) activity in the curative and protective assays. The concentrations of jasmonic acid (JA) and jasmonic acid -isoleucine (JA-Ile) and the expression of its key gene NtCOI1 in Tag A-treated and systemic leaves of treated plants were significantly higher than those of the control plant. Furthermore, Tag A-induced resistance to TSWV could be eliminated by VIGS-mediated silencing of the NtCOI1 gene. These indicated that Tag A acts against TSWV by activating the JA defense signaling pathway.

中文翻译：

来自 Tithonia diversifolia 的 Tagitinin A 通过诱导系统抗性提供对番茄斑枯病原孢病毒的抗性

番茄斑萎病原孢病毒 (TSWV) 对全世界的农艺和观赏作物造成毁灭性的损失。目前，没有有效的策略来控制这种疾病。使用生物诱导剂增强植物对病毒的抗性可能是一种有效的方法。我们之前的研究表明 Tagitinin A (Tag A) 对 TSWV 具有很高的治疗和保护作用。然而，标签 A 介导的抗病毒活性的潜在分子机制仍然未知。在这项研究中，标签 A 降低了 NSs 的表达，TSWV 感染后未经处理的叶子中 NSm 基因的表达非常低。此外，在保护性试验中，接种和系统叶片中所有TSWV基因的表达均受到抑制，并且在系统叶片中抑制率超过85%。标签 A 在治疗和保护试验中增加苯丙氨酸解氨酶 (PAL) 活性。Tag A处理和系统叶片中茉莉酸(JA)和茉莉酸-异亮氨酸(JA-Ile)浓度及其关键基因NtCOI1的表达显着高于对照植物。此外，可以通过 VIGS 介导的 NtCOI1 基因沉默来消除 Tag A 诱导的 TSWV 抗性。这些表明标签 A 通过激活 JA 防御信号通路来对抗 TSWV。标签 A 诱导的 TSWV 抗性可以通过 VIGS 介导的 NtCOI1 基因沉默来消除。这些表明标签 A 通过激活 JA 防御信号通路来对抗 TSWV。标签 A 诱导的 TSWV 抗性可以通过 VIGS 介导的 NtCOI1 基因沉默来消除。这些表明标签 A 通过激活 JA 防御信号通路来对抗 TSWV。

更新日期：2020-10-01

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