X-ray CT imaging can be complementary to fluorescence and photoacoustic imaging (FLI and PAI), allowing for high spatial resolution and high-sensitivity multimodal imaging for imaging guided treatment. In this study, the CT contrast agent iohexol was co-encapsulated with indocyanine green (ICG) within nanoliposomes (NLs) to explore their interaction and possible application of this liposomal formulation (LGI) in cancer theranostics. The photophysical properties of LGI were studied to assess the effect of iohexol on ICG that can enhance the efficiency of ICG-based near infrared photodynamic therapy (PDT). The CT, FLI and PA imaging abilities of LGI were also investigated. Furthermore, the near infrared phototherapy of cancer cells in vitro was performed, exhibiting higher phototherapy efficacy of LGI in comparison with other ICG formulations. We conclude that LGI can serve as a highly efficient theranostic nanoplatform for multimodal (fluorescence, CT and PA) imaging and near infrared phototherapy.

X射线CT成像可以与荧光和光声成像（FLI和PAI）互补，从而为成像引导治疗提供高空间分辨率和高灵敏度多峰成像。在这项研究中，CT造影剂碘海醇与吲哚菁绿（ICG）共封装在纳米脂质体（NLs）中，以研究它们的相互作用以及这种脂质体制剂（LGI）在癌症治疗中的可能应用。研究了LGI的光物理性质，以评估碘海醇对ICG的作用，该作用可增强基于ICG的近红外光动力疗法（PDT）的效率。还研究了LGI的CT，FLI和PA成像能力。此外，进行了癌细胞的体外近红外光疗，与其他ICG制剂相比，LGI具有更高的光疗功效。