To enhance the qualitative bacterial biomass per unit of media and to overcome the limitations of the existing haemorrhagic septicaemia (HS) vaccines, a comprehensive study was undertaken encompassing the role of iron on the bacterial biomass of Pasteurella multocida B: 2 to vaccine development. Trypsin digested hydrochloric acid-treated sheep blood (THSB) as a novel iron rich supplement had been devised for the first time for augmenting the qualitative bacterial biomass per unit of media which was evident with growth kinetic study. The higher recovery of iron from THSB became evident via atomic absorbance spectrophotometry. The critical level of iron in the media as well as mode of iron supplementation showed a major impact on the outer membrane protein profile of P. multocida B:2 and variation in droplet size and particle-size distribution of formulated vaccine. Immune response study against iron-regulated bacterin adjuvanted with aluminum hydroxide gel in mouse model showed that 3% THSB supplementation of casein sucrose yeast (CSY) not only augmented the growth of P. multocida B:2 significantly but conferred highest pre-challenged ELISA IgG titer and protection against pasteurellosis. Thus, THSB supplementation of CSY can resolve existing up-scaling and immunogenic potential problems of HS vaccine production.

为了提高单位培养基中细菌的定性细菌生物量，并克服现有的出血性败血性（HS）疫苗的局限性，进行了一项全面的研究，其中包括铁在多杀巴斯德氏菌B：2细菌生物量中对疫苗开发的作用。胰蛋白酶消化的盐酸处理的绵羊血（THSB）作为一种新型的富含铁的补充剂，已被首次设计用于增加单位培养基中细菌的生物量，这在生长动力学研究中很明显。通过原子吸收分光光度法可以明显地从THSB中回收铁。培养基中铁的临界水平以及铁的添加方式显示出对铁膜外膜蛋白质谱的重大影响P. multocida B：2和配制疫苗的液滴大小和粒径分布变化。在小鼠模型中对辅以铁调节的细菌辅以氢氧化铝凝胶进行的免疫反应研究表明，酪蛋白蔗糖酵母（CSY）的3％THSB补充不仅显着增加了多杀性巴氏杆菌B：2的生长，而且赋予了最高的挑战前ELISA IgG滴度和抗巴氏杆菌病的保护作用。因此，补充CSY的THSB可以解决HS疫苗生产中现有的扩大规模和潜在的免疫原性问题。