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Ascorbic acid is essential for inducing chromium (VI) toxicity tolerance in tomato roots.
Journal of Biotechnology ( IF 4.1 ) Pub Date : 2020-07-15 , DOI: 10.1016/j.jbiotec.2020.07.011
Asma A Al-Huqail 1 , Hayssam M Ali 1 , Bishwajit Kumar Kushwaha 2 , Arwa A Al-Huqail 3 , Vijay Pratap Singh 2 , Manzer H Siddiqui 1
Affiliation  

Problem of chromium (Cr) pollution is of great scientific concern as it adversely affects crop productivity worldwide. Therefore, scientific efforts are being made to minimize Cr toxicity in crop plants by using various methods. Of these methods, use of certain chemicals like ascorbic acid (ASC), glutathione, proline, nutrients, etc. has shown promising results. Therefore, in this study, we have tested a role of ASC in regulating hexavalent chromium [Cr(VI)] toxicity in tomato roots. Chromium (VI) reduced length, dry weight, fitness and tissue density of roots due to enhanced cellular accumulation of Cr which leads to the cell death. Chromium (VI) also declined ASC pool and activity of its regenerating enzymes along with enhanced level of oxidative stress and damage to lipids and proteins. However, exogenous addition of ASC significantly reversed toxic effects of Cr(VI) in tomato roots. Furthermore, addition of lycorine (inhibitor of ASC biosynthesis) interestingly augmented Cr(VI) toxicity. However, exogenous addition of ASC reversed toxic effect of lycorine suggesting that endogenous ASC has role in alleviating Cr(VI) toxicity in tomato roots.



中文翻译:

抗坏血酸对于诱导番茄根中的铬(VI)毒性耐受性至关重要。

铬(Cr)污染问题是科学界极为关注的问题,因为它对全世界的农作物生产力产生不利影响。因此,正在进行科学努力,以通过使用各种方法使农作物中的Cr毒性最小化。在这些方法中,使用某些化学物质如抗坏血酸(ASC),谷胱甘肽,脯氨酸,营养物质等已显示出令人鼓舞的结果。因此,在这项研究中,我们测试了ASC在调节番茄根中六价铬[Cr(VI)]毒性中的作用。铬(VI)减少了根的长度,干重,适应度和组织密度,这是由于Cr的细胞积累增加,导致细胞死亡。铬(VI)还降低了ASC池和其再生酶的活性,同时增加了氧化应激水平以及对脂质和蛋白质的破坏。然而,外源添加ASC可以显着逆转番茄根中Cr(VI)的毒性作用。此外,添加lycorine(ASC生物合成抑制剂)有趣地增强了Cr(VI)毒性。但是,外源添加ASC逆转了赖氨酸的毒性作用,表明内源ASC在减轻番茄根中的Cr(VI)毒性中具有作用。

更新日期:2020-07-22
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