The silver stripped skipper, Leptalina unicolor, is listed as an endangered species with a paucity of leftover populations in Korea. Prior population genetic analysis is essential for the establishment of effective conservation strategies. In the present study, we investigated the genetic diversity and the relationships of L. unicolor populations in Korea using 12 newly developed L. unicolor-specific microsatellite markers and two mitochondrial DNA (mtDNA) sequences (a total of 1283 bp from COI and CytB). Due to the endangered status and rarity, a total of 33 individuals from two localities in Gangwon-do Province (Seohwa and Girin) and one in Gyeongsangnam-do Province (Danjang) were sampled. The concatenated mtDNA sequences revealed very low genetic diversity in each population, with only one to three independent haplotypes per population. F_ST, principal coordinate analysis, and independent structural analysis of the concatenated mtDNA sequences and the 12 microsatellite loci developed in this study showed that each L. unicolor population in Korea is nearly completely isolated, although inbreeding has not yet occurred. Long-term conservation habitat recovery that increases the available population appears to be important in the prevention of genetic drift and inbreeding, which occur in small isolated populations and reduce the viability of populations under fluctuating environmental conditions.

银色的船长Leptalina unicolor被列为濒临灭绝的物种，在韩国的剩余人口很少。事先进行种群遗传分析对于建立有效的保护策略至关重要。在本研究中，我们使用12个新开发的单色单色微卫星标记和两个线粒体DNA（mtDNA）序列（来自COI和CytB的共1283 bp），调查了韩国单色盲种群的遗传多样性和亲缘关系。）。由于濒临灭绝的地位和稀有性，从江原道省的两个地方（Seohwa和Girin）和庆尚南道省的一个地方（Danjang）总共采样了33个人。串联的mtDNA序列显示每个人群的遗传多样性非常低，每个人群只有一到三个独立的单倍型。这项研究开发的串联mtDNA序列和12个微卫星基因座的F _ST，主坐标分析和独立结构分析表明，每个L. unicolor尽管近亲繁殖尚未发生，但韩国的人口几乎完全是孤立的。长期保护生境的恢复会增加可用种群，这对于预防遗传漂移和近亲繁殖很重要，因为遗传漂移和近亲繁殖发生在孤立的小种群中，并在波动的环境条件下降低了种群的生存力。