Abstract This study tested multiple methods for assessing microbial populations and identified bacteria detected on supermarket food-contact surfaces. The results for culture medium–based conventional methods, i.e., swabbing, direct count, sampling discs, and Petrifilm, showed that surface cleaning was satisfactory. However, direct epifluorescence microscopy (DEM) and bioluminescence detection of adenosine triphosphate (ATP) showed that surface cleaning was unsatisfactory. The surfaces of the fruit and vegetables section were the most contaminated, followed by the sections for cured meats and cheeses, fresh seafood, the aisles of the sections tested, meat, and yogurts and desserts. Likewise, significantly higher microbiological counts were observed on painted wood than on stainless steel, plastic, and painted epoxy surfaces (p

中文翻译：

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使用多种采样方法评估细菌种群并鉴定超市食品接触表面上检测到的细菌

摘要 本研究测试了多种评估微生物种群的方法，并确定了在超市食品接触表面检测到的细菌。以培养基为基础的常规方法（即擦拭、直接计数、取样盘和 Petrifilm）的结果表明，表面清洁效果令人满意。然而，直接落射荧光显微镜 (DEM) 和三磷酸腺苷 (ATP) 的生物发光检测表明表面清洁效果不理想。水果和蔬菜部分的表面污染最严重，其次是腌肉和奶酪、新鲜海鲜、测试部分的过道、肉类、酸奶和甜点。同样，在涂漆木材上观察到的微生物计数明显高于不锈钢、塑料和涂漆环氧树脂表面（p