The ciprofloxacin (CIP) abuse has caused many problems threatening to human health. Here, we design the quantum dot microsphere (QDM) based immunochromatographic quantitative CIP test strip: when the sample under detection contains CIP, the QDM-monoclonal antibody (mAb) probes bound with the CIP and cannot be captured by CIP-bovine serum albumin (BSA) conjugation dispersed on the T lines, reducing the fluorescence intensities. These test strips can provide a low detection limit of 0.05 ng/mL and a wide linear detection range from 0.1 ng/mL to 100 ng/mL in high sensitivity and accuracy as well as good selectivity, reproducibility and stability. Moreover, a smartphone based test strip reader with the size of 85 mm × 48 mm × 44 mm is also fabricated using 3-D printing to automatically and quantitatively detect CIP. The whole process of CIP detection can be finished within 15 min, but only cost ~1 RMB (10 cents).

环丙沙星（CIP）的滥用已引起许多威胁人类健康的问题。在这里，我们设计了基于量子点微球（QDM）的免疫色谱定量CIP测试条：当被检测的样品包含CIP时，与CIP结合的QDM单克隆抗体（mAb）探针无法被CIP-牛血清白蛋白捕获（ BSA）共轭分散在T线上，降低了荧光强度。这些测试条可提供0.05 ng / mL的低检测限，并具有从0.1 ng / mL到100 ng / mL的宽线性检测范围，从而具有高灵敏度和准确性，以及良好的选择性，可重复性和稳定性。此外，还使用3-D打印技术制造了基于智能手机的试纸条读取器，尺寸为85 mm×48 mm×44 mm，以自动定量检测CIP。