Introduction

It has been reported that microbiological diagnosis of Cutibacterium spp. infection requires a prolonged incubation time (up to 14 days). We present our experience with regard to incubation time for detection of Cutibacterium spp. in orthopaedic samples over a 10-year period.

Methods

One hundred and nineteen samples were included in this retrospective study. Fifty-three were implants (having previously undergone sonication), 64 were periprosthetic tissue biopsies and two were synovial fluids. Atkins's criteria were used for interpreting the isolates. Quantification and number of days until a culture became positive for Cutibacterium spp. were evaluated.

Results

The median number of days to detection of a clinically significant isolate and a contaminant was 4 days. No clinically significant isolates grew after day eight.

Conclusion

Most clinically significant isolates of Cutibacterium spp. are detected in the first 7 days of incubation, although a recommendation of prolonged incubation (up to 14 days) appears to be necessary for detecting other organisms.

中文翻译：

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角质杆菌属 从骨科植入物相关感染中分离出来：一种生长不那么缓慢的有机体

介绍

据报道，角质杆菌属的微生物学诊断。感染需要较长的潜伏期（最多 14 天）。我们介绍了我们在检测角质杆菌属的孵育时间方面的经验。在超过 10 年的骨科样本中。

方法

该回顾性研究包括 119 个样本。53 个是植入物（之前经过超声处理），64 个是假体周围组织活检，2 个是滑液。Atkins 标准用于解释分离株。培养物成为角质杆菌属阳性之前的定量和天数。被评估。

结果

检测到具有临床意义的分离株和污染物的平均天数为 4 天。第八天后没有临床显着的分离株生长。

结论

最有临床意义的角质杆菌属分离株。潜伏期的前 7 天检测到，尽管建议延长潜伏期（最多 14 天）对于检测其他生物体似乎是必要的。