Abstract

To develop low-ODAP grass pea genotypes with high protein content, in vitro tissue culture techniques were used for inducing somaclonal variation through internode explants that was cultured on B5 medium containing 2 mg L^− l α-naphthaleneacetic acid and 1 mg L^− l 6-benzylaminopurine of 19 grass pea genotypes. The selected somaclones (R₃ and R₄ generations) were evaluated for variation in ODAP, protein, and quantitative agronomic traits. The results showed statistically significant (P < 0.001) genotypic differences for ODAP %, Protein %, 100-seed weight (HSW) and pod width (PODW) in the R₄ somaclones under field conditions. The somaclones showed broad-sense heritability estimate of 0.14 ± 0.064 for ODAP%, 0.87 ± 0.015 for protein, 0.78 ± 0.025 for HSW and 0.13 ± 0.062 for PODW. Among the 270 somaclones, Sel462-5 was significantly superior to the parent and other somaclones in terms of ODAP content (0.13%), protein content (28.47%) and pod width (1.43 cm). The correlation coefficients between parents and somaclones for pod width with 100-seed weight, ODAP-clones and protein-clones were positive and significant. The superiority of somaclones for ODAP, protein, 100-seed weight and pod width in R₄ generation opened the possibility for grass pea improvement through somaclonal variation.

中文翻译：

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在19种草豌豆（Lathyrus sativus L.）基因型中，培养物选择了显示低ODAP和高蛋白含量的体细胞克隆变异体

摘要

为了开发具有高蛋白质含量的低ODAP草豌豆基因型，使用体外组织培养技术通过节间外植体诱导体细胞克隆变异，将其培养在含有2 mg L ⁻ lα-萘乙酸和1 mg L ^{− l} 6的B5培养基上-19种豌豆基因型的苄基氨基嘌呤。评价所选的体细胞克隆体（R ₃和R ₄代）的ODAP，蛋白质和定量农艺性状的变异。结果显示R _4中ODAP％，蛋白质％，100粒重（HSW）和豆荚宽度（PODW）的基因型差异具有统计学显着性（P <0.001）田间条件下的松果体。松果体的广义遗传力估计值对于ODAP％为0.14±0.064，对于蛋白质为0.87±0.015，对于HSW为0.78±0.025，对于PODW为0.13±0.062。在270个体细胞克隆中，Sel462-5在ODAP含量（0.13％），蛋白质含量（28.47％）和豆荚宽度（1.43 cm）方面显着优于亲本和其他体细胞克隆。亲本和体细胞无性系之间的荚果宽度与100粒重，ODAP克隆和蛋白质克隆之间的相关系数为正且显着。R ₄代中体细胞克隆在ODAP，蛋白质，100粒重和荚果宽度方面的优势为通过体细胞克隆变异改良豌豆提供了可能性。