Aiming to develop specific diagnostic tool for invasive fungal infection, a radiopharmaceutical based on anidulafungin was investigated. A two-step approach was used to label the drug with technetium-99m to retain its biological activity. 99mTc-tricarbonyl-Anidulafugin complex was obtained in high radiochemical yield (~98%). The complex was radio-chemically characterized and physico-chemically evaluated to assess its suitability as a radiopharmaceutical. Thereafter, it was evaluated in vitro and in vivo using different models; sterile inflammation, bacterial and fungal infection in mice and showed specific accumulation [C. albicans model (~6.5%), A. fumigatus model (~5.2%)]. All results supported its usefulness as specific tracer for fungal infection.

中文翻译：

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99mTc-阿尼芬净的制备和评价：一种潜在的真菌感染放射性示踪剂

为了开发侵袭性真菌感染的特异性诊断工具，研究了一种基于阿尼芬净的放射性药物。使用两步法用锝99m 标记药物以保持其生物活性。以高放射化学产率 (~98%) 获得了 99mTc-三羰基-Anidulafugin 复合物。对该复合物进行放射化学表征和物理化学评估，以评估其作为放射性药物的适用性。此后，使用不同的模型进行了体外和体内评估；小鼠无菌炎症、细菌和真菌感染并表现出特异性积累 [C. 白色念珠菌模型（~6.5%），烟曲霉模型（~5.2%）]。所有结果都支持其作为真菌感染的特异性示踪剂的有用性。