In the present work, we developed and validated five spectrophotometric methods for determination of magnesium orotate dihydrate in distilled water, pH 1.2, 4.5, 6.8, and 7.4 buffer systems. These media were selected to extend the applicability of the developed methods from the development of dosage forms to regular quality control. In all media, linear correlation was observed over the concentration range from 1 to 25 μg/mL. The developed methods were found to be selective and specific to the determination of magnesium orotate dihydrate in the presence of various excipients, as well as accurate, precise, and robust (the percent relative standard deviation was found to be less than 2%, and the percent recoveries were between 98 to 102%). The developed methods were also found to be highly sensitive, since the detection and quantification limits were found to be less than 1 μg/mL. The degradation kinetics studies reveal the stability of magnesium orotate dihydrate against hydrolysis (acidic or basic), oxidation, photolysis, and thermal treatment.

中文翻译：

---

光谱法研究二水合氢氧化镁的降解动力学

在目前的工作中，我们开发并验证了五种分光光度法，用于测定pH 1.2、4.5、6.8和7.4缓冲液中蒸馏水中的乳清酸镁二水合物。选择这些培养基以将开发方法的适用性从剂型开发扩展到常规质量控制。在所有介质中，在1至25μg/ mL的浓度范围内均观察到线性相关。发现开发的方法对于在各种赋形剂存在下测定乳清酸二水合镁具有选择性和特异性，以及准确，精确和可靠（相对标准偏差百分比小于2％，并且回收率介于98％到102％之间）。还发现开发的方法非常敏感，因为发现的检测和定量限小于1μg/ mL。降解动力学研究揭示了乳清酸镁二水合物对水解（酸性或碱性），氧化，光解和热处理的稳定性。