Echinacea (Echinacea purpurea L.), also known as purple coneflower, is one of the important medicinal plants commonly used for respiratory infections. The aim of this study was to compare different transgenic hairy root lines for growth and secondary metabolite biosynthesis and to select a superior hairy root line for the production of caffeic acid derivatives (CADs). Agrobacterium rhizogenes-mediated root lines showing hairy root phenotypic characteristics were analyzed by the Southern blot analysis and the copy number of the rolC gene determined. Significant differences were found among seven hairy root lines evaluated for the copy number of rolC gene, root growth, total phenolic, and CAD contents. It was concluded that these differences may be due to the position and copy number of rolC gene in the genome. Across the parameters compared, it was found that line 7 was superior to the other transgenic lines. The results show that the selection of superior transgenic lines is an important factor for the efficient production of secondary metabolites in hairy root cultures of Echinacea.

紫锥菊（紫锥菊），也称为紫色锥花，是通常用于呼吸道感染的重要药用植物之一。这项研究的目的是比较不同的转基因毛状根系的生长和次生代谢产物的生物合成，并选择一种优良的毛状根系来生产咖啡酸衍生物（CAD）。通过Southern印迹分析来分析发根农杆菌介导的显示毛状根表型特征的根系，并确定rol C基因的拷贝数。在对rolC拷贝数进行评估的7个毛状根系中发现了显着差异基因，根生长，总酚和CAD含量。结论是这些差异可能是由于rolC基因在基因组中的位置和拷贝数所致。在所比较的参数中，发现品系7优于其他转基因品系。结果表明，优良的转基因品系的选择是紫锥菊的毛状根培养物中有效产生次生代谢产物的重要因素。