Pre-treatment of biomass is of great importance to maximize the recovery of fermentable sugars and minimize the amount of enzyme inhibitors formed. There are several processes that can be applied for pre-treatment of agricultural industrial waste such as chemical, physical, physico-chemical, biological, and combined pre-treatments. Alkaline hydrogen peroxide (AHP) and ultrasonication are two of this pre-treatment process. The aim of this study was to investigate the pre-treatment performance of barley straw biomass using AHP and combined ultrasonic-hydrogen peroxide process via Box-Behnken design as a response surface methodology (RSM). The factors of time (30–120 min), particle size (0.2–1 mm), peroxide dosage (1–5%), and temperature (50–100 °C) are investigated for optimum operating conditions. For ultrasonication process, factors are time (5–30 min), particle size (0.2–1.0 mm) and US power (20–100 Kj/kgDM). During AHP pre-treatment, maximum total and reduced sugar concentration was found as 14 g/L and 660 mg/L, respectively, at maximum level of time and minimum level of dosage factors which are 120 min and 1%, respectively. Maximum total and reduced sugar concentration can be reached nearly 7.6 g/L and 810 mg/L, respectively, at minimum level of time which is 5 min and almost minimum level of chemical dosage in ultrasound-hydrogen peroxide process, respectively.

生物质的预处理对于最大程度地提高可发酵糖的回收率并最小化形成的酶抑制剂的数量非常重要。有几种方法可用于农业工业废物的预处理，例如化学，物理，物理化学，生物和联合预处理。碱性过氧化氢（AHP）和超声处理是该预处理过程中的两种。这项研究的目的是通过Box-Behnken设计作为响应面方法（RSM），研究使用AHP和超声-过氧化氢联合工艺对大麦秸秆生物质进行预处理的性能。对于最佳操作条件，研究了时间因素（30–120分钟），粒径（0.2–1毫米），过氧化物的用量（1-5％）和温度（50–100°C）。对于超声波处理，影响因素包括时间（5-30分钟），粒径（0.2-1.0毫米）和美国功率（20-100 Kj / kgDM）。在AHP预处理过程中，最大时间和最小剂量因子分别为120分钟和1％，最大总糖浓度和降低的糖浓度分别为14 g / L和660 mg / L。在5分钟的最小时间水平和超声-过氧化氢工艺中化学剂量的几乎最低水平下，最大总糖浓度和降低的糖浓度分别可以达到近7.6 g / L和810 mg / L。