Design of a FRET-based fluorescent probe for the reversible detection of SO2 and formaldehyde in living cells and mice,New Journal of Chemistry

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Design of a FRET-based fluorescent probe for the reversible detection of SO2 and formaldehyde in living cells and mice
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2020-07-14 , DOI: 10.1039/d0nj03071f
Wenjie Gao _{1,

2,

3,

4,

5} , Yanyan Ma _{1,

2,

3,

4,

5} , Weiying Lin _{1,

2,

3,

4,

5}

Affiliation

SO₂ and formaldehyde (FA) are important signal molecules in physiological and pathological processes. It is particularly urgent to seek a method that can monitor SO₂ and FA in real-time to study their role in organisms. Herein, we designed and synthesized a ratiometric fluorescent probe NaP based on the FRET mechanism for the reversible detection of SO₂ and FA. This probe possesses high sensitivity, good selectivity, excellent photostability, low cytotoxicity and an ultra-fast response to SO₂ (within 5 s). Notably, the probe can be recovered efficiently by FA. In addition, the biological experiments showed that the novel probe NaP could reversibly image SO₂ and FA in cells and mice.

中文翻译：

基于FRET的荧光探针的设计，用于可逆检测活细胞和小鼠中的SO2和甲醛

SO ₂和甲醛（FA）是生理和病理过程中的重要信号分子。寻找一种可以实时监测SO ₂和FA的方法以研究其在生物中的作用尤为迫切。本文中，我们基于FRET机制设计和合成了比例荧光探针NaP，用于可逆检测SO ₂和FA。该探针具有高灵敏度，良好的选择性，优异的光稳定性，低细胞毒性和对SO ₂的超快速响应（在5 s内）。值得注意的是，该探针可以通过FA有效地回收。此外，生物学实验表明，新型探针NaP可以可逆成像SO ₂ 和细胞和小鼠中的FA。

更新日期：2020-08-17

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