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Design of a FRET-based fluorescent probe for the reversible detection of SO2 and formaldehyde in living cells and mice
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2020-07-14 , DOI: 10.1039/d0nj03071f
Wenjie Gao 1, 2, 3, 4, 5 , Yanyan Ma 1, 2, 3, 4, 5 , Weiying Lin 1, 2, 3, 4, 5
Affiliation  

SO2 and formaldehyde (FA) are important signal molecules in physiological and pathological processes. It is particularly urgent to seek a method that can monitor SO2 and FA in real-time to study their role in organisms. Herein, we designed and synthesized a ratiometric fluorescent probe NaP based on the FRET mechanism for the reversible detection of SO2 and FA. This probe possesses high sensitivity, good selectivity, excellent photostability, low cytotoxicity and an ultra-fast response to SO2 (within 5 s). Notably, the probe can be recovered efficiently by FA. In addition, the biological experiments showed that the novel probe NaP could reversibly image SO2 and FA in cells and mice.

中文翻译:

基于FRET的荧光探针的设计,用于可逆检测活细胞和小鼠中的SO2和甲醛

SO 2和甲醛(FA)是生理和病理过程中的重要信号分子。寻找一种可以实时监测SO 2和FA的方法以研究其在生物中的作用尤为迫切。本文中,我们基于FRET机制设计和合成了比例荧光探针NaP,用于可逆检测SO 2和FA。该探针具有高灵敏度,良好的选择性,优异的光稳定性,低细胞毒性和对SO 2的超快速响应(在5 s内)。值得注意的是,该探针可以通过FA有效地回收。此外,生物学实验表明,新型探针NaP可以可逆成像SO 2 和细胞和小鼠中的FA。
更新日期:2020-08-17
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