Identification of molecular characterization of genes underlying livestock productive traits may allow applying advanced biotechnology techniques to improve animal productivity. Growth hormone (GH) controls body growth rate, milk production, reproduction as well as carbohydrate, lipid, and protein metabolism. Therefore, the present study aims to investigate the genetic variations of growth hormone cDNA sequences between Assaf sheep (As_GH) and Boer goat (Bo_GH) that mainly used for genetic improvement in Egypt using bioinformatics analysis. Growth hormone cDNA was isolated from the pituitary gland tissue of Assaf sheep Boer goat and subcloned into pTZ57R/T cloning vector for sequencing. Molecular weight of As_GH cDNA was 665 bp and was 774 bp for Bo_GH cDNA. The complete coding sequences (CDS) of As_GH and Bo_GH were registered in the GenBank database under accession number (AC: MH128986 and AC: MG744290, respectively). High homology percentage was observed (99.5%) between AS_GH and Bo_GH protein sequences with one different amino acid in the As_GH protein sequence (Arg194). The protein sequence of As_GH has only one motif signature; Somatotropin_1 from 79 to 112 aa compared to Bo_GH protein sequences and GenBank database that had two motifs signature. The growth hormone cDNA sequence of Assaf sheep has a unique three single nucleotide polymorphisms (SNPs) (A637A638G639) that encodes for arginine (Arg194); this insertion mutation (AAG) was not found in the growth hormone cDNA sequences of Boer goat in the present study and GenBank database breeds. This mutation can be used to develop SNPs markers for Assaf sheep. GH sequence of Assaf and Boer goat is highly conserved and the homogeny in the codon region (99.5%). The Assaf sheep GH sequence has a unique three SNPs that may be used to develop SNPs markers for such breed. Further studies are needed to investigate the genetic variations of growth hormone gene in different sheep and goat breeds in Egypt and document the relationship between these variations and the productive performance of animals.

中文翻译：

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埃及饲养的阿萨夫绵羊和波尔山羊生长激素 mRNA 的克隆、核苷酸测序和生物信息学分析。

鉴定牲畜生产性状基因的分子特征可能允许应用先进的生物技术来提高动物生产力。生长激素 (GH) 控制身体生长速度、产奶量、繁殖以及碳水化合物、脂质和蛋白质代谢。因此，本研究旨在利用生物信息学分析研究主要用于埃及遗传改良的阿萨夫绵羊（As_GH）和波尔山羊（Bo_GH）之间生长激素cDNA序列的遗传变异。从阿萨夫绵羊、波尔山羊的垂体组织中分离出生长激素cDNA，亚克隆到pTZ57R/T克隆载体中进行测序。As_GH cDNA 的分子量为665 bp，Bo_GH cDNA 的分子量为774 bp。As_GH和Bo_GH的完整编码序列(CDS)在GenBank数据库中注册，登录号分别为AC：MH128986和AC：MG744290。在 AS_GH 和 Bo_GH 蛋白质序列之间观察到高同源性百分比 (99.5%)，其中 As_GH 蛋白质序列 (Arg194) 中有一个不同的氨基酸。As_GH 的蛋白质序列只有一个基序特征；与具有两个基序特征的 Bo_GH 蛋白质序列和 GenBank 数据库相比，Somatotropin_1 从 79 个氨基酸到 112 个氨基酸。阿萨夫羊的生长激素 cDNA 序列具有独特的三个单核苷酸多态性 (SNP) (A637A638G639)，编码精氨酸 (Arg194)；在本研究和GenBank数据库品种的波尔山羊生长激素cDNA序列中均未发现该插入突变(AAG)。该突变可用于开发 Assaf 羊的 SNP 标记。阿萨夫山羊和波尔山羊的GH序列高度保守，密码子区域同质性(99.5%)。Assaf 绵羊 GH 序列具有独特的三个 SNP，可用于开发此类品种的 SNP 标记。需要进一步的研究来调查埃及不同绵羊和山羊品种中生长激素基因的遗传变异，并记录这些变异与动物生产性能之间的关系。