Entomopathogenic nematodes (EPNs) are being used for the management of insect pests occurring in both agricultural and horticultural crops. But native EPNs are reasonably more efficacious in controlling insect pests than introduced EPNs species because they are adapted to local environmental conditions and insect pests. Therefore, in the present study, a survey was conducted to isolate EPNs at 4 districts of Purvanchal Region and 1 district of Bundelkhand Region of Uttar Pradesh, India, between 2016 and 2017 years. Out of 130 soil samples, EPNs were recovered from 3 soil samples (2.3%). Morphological characters examination and ITS-rDNA region revealed that the 3 EPNs such as Steinernema sp. (IIVR JNC01 strain) Steinernema sp. (IIVR JNC02 strain), and Steinernema sp. (IIVR EPN03 strain) are belonging to Steinernema siamkayai. Further pathogenicity of all these strains was tested on Galleria mellonella Linnaeus and the most effective strain was used to determine the biocontrol potential against lepidopteran and coleopteran pests of major vegetable crops in comparison to commercially available Heterorhabditis indica (NBAIIH38 strain). Results revealed that S. siamkayai (IIVR JNC01 strain) caused 100, 100, and 85%, mortality of 3rd instar larvae of Spodoptera litura Fabricius, Spilosoma obliqua Walker, and Spoladea recurvalis Fabricius, respectively. Similarly, S. siamkayai (IIVR JNC01 strain) caused 92.5% mortality of 2nd instar grubs of Myllocerus subfaciatus Gurein under laboratory conditions. The present study revealed that EPNs commonly present at Purvanchal and Bundelkhand regions of Uttar Pradesh and S. siamkayai (IIVR JNC01 strain) isolated from the Bundelkhand Region showed a good biocontrol potential against major insect pests of vegetable crops.

中文翻译：

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印度北方邦Purvanchal和Bundelkhand地区发生的昆虫病原线虫的分离，鉴定和生物防治潜力

昆虫病原线虫（EPN）正用于管理农业和园艺作物中的害虫。但是，本地EPNs在控制虫害方面比引进的EPNs物种更有效，因为它们适应了当地的环境条件和害虫。因此，在本研究中，进行了一项调查以分离2016年至2017年之间印度北方邦Purvanchal地区的4个地区和Bundelkhand地区的1个地区的EPN。在130个土壤样品中，从3个土壤样品中回收了EPN（2.3％）。形态特征检查和ITS-rDNA区域显示3个EPN，如Steinernema sp.。（IIVR JNC01菌株）Steinernema sp。（IIVR JNC02株）和Steinernema sp。（IIVR EPN03菌株）属于Steinernema siamkayai。所有这些菌株的进一步致病性均在Malleria mellonella Linnaeus上进行了测试，并且与市售的Heterorhabditis indica（NBAIIH38菌株）相比，最有效的菌株用于确定对主要蔬菜作物的鳞翅目和鞘翅目害虫的生物防治潜力。结果显示，S。siamkayai（IIVR JNC01株）分别导致斜纹夜蛾，斜纹螺和沃氏S三龄幼虫的死亡率分别为100％，100％和85％。类似地，在实验室条件下，S。siamkayai（IIVR JNC01株）导致了亚黑粉病菌的第二龄幼虫的92.5％死亡率。本研究表明，EPT通常存在于北方邦和S.的Purvanchal和Bundelkhand地区。