The SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its Receptor Binding Domain in two conformations: receptor-accessible "up" or receptor-inaccessible "down" conformations. Here, we report that the commonly used stabilized S ectodomain construct "2P" is sensitive to cold temperature, and that this cold sensitivity is resolved in a "down" state stabilized spike. Our results will impact structural, functional and vaccine studies that use the SARS-CoV-2 S ectodomain.

中文翻译：

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SARS-CoV-2 刺突胞外域的冷敏感性。

SARS-CoV-2 刺突 (S) 蛋白是 COVID-19 疫苗开发的主要目标，它的受体结合域具有两种构象：受体可接近的“向上”或受体不可接近的“向下”构象。在这里，我们报告了常用的稳定 S ectodomain 构造“2P”对低温敏感，并且这种冷敏感性在“向下”状态稳定尖峰中得到解决。我们的结果将影响使用 SARS-CoV-2 Sectodomain 的结构、功能和疫苗研究。