Rapid and Sensitive Immunochromatographic Method-Based Monoclonal Antibody for the Quantitative Detection of Metalaxyl in Tobacco.,ACS Omega

当前位置： X-MOL 学术 › ACS Omega › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Rapid and Sensitive Immunochromatographic Method-Based Monoclonal Antibody for the Quantitative Detection of Metalaxyl in Tobacco.
ACS Omega ( IF 3.7 ) Pub Date : 2020-07-13 , DOI: 10.1021/acsomega.0c01782
Ping Ni ₁ , Liqiang Liu ₁ , Changlong Hao ₁ , Xinxin Xu ₁ , Shanshan Song ₁ , Hua Kuang ₁ , Chuanlai Xu ₁

Affiliation

In this study, we prepared a monoclonal antibody (mAb) against metalaxyl (Met) with a half-maximum inhibitory concentration (IC₅₀) of 0.54 ng/mL based on a new hapten, and a gold nanoparticle-based immunochromatographic assay (GICA) was developed for the rapid detection of Met residues in tobacco. Under optimal conditions, even with the naked eye, one can see the semiquantitative analysis results. The naked eye detection limit of Met in tobacco is 25 μg/kg, and the detection threshold is 100 μg/kg. In addition, the cross-reactivity test shows that the mAb has good specificity for Met, and the GICA results have a good correlation with the indirect competitive enzyme-linked immunosorbent assay and liquid chromatography with tandem mass spectrometry test results, which show that the method is feasible and reliable and are more convenient and quicker than the methods using instrumentation for detection. Therefore, GICA may provide a useful tool for the rapid screening and detection of Met residues in tobacco.

中文翻译：

基于快速灵敏的免疫色谱方法的单克隆抗体，用于烟草中甲霜灵的定量检测。

在这项研究中，我们制备了针对甲霜灵（Met）的单克隆抗体（mAb），其半数抑制浓度为（IC ₅₀浓度为0.54 ng / mL的新半抗原为基础，开发了一种基于金纳米颗粒的免疫色谱分析（GICA），用于快速检测烟草中的Met残留。在最佳条件下，即使用肉眼也可以看到半定量分析结果。烟草中Met的肉眼检测极限为25μg/ kg，检测阈值为100μg/ kg。此外，交叉反应测试表明mAb对Met具有良好的特异性，GICA结果与间接竞争酶联免疫吸附测定和液相色谱-串联质谱测试结果具有良好的相关性，表明该方法与使用仪器进行检测的方法相比，它既可行又可靠，并且更加方便快捷。因此，

更新日期：2020-07-28

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11