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Development of an ic-ELISA and Immunochromatographic Strip Assay for the Detection of Diacetoxyscirpenol in Rice.
ACS Omega ( IF 3.7 ) Pub Date : 2020-07-13 , DOI: 10.1021/acsomega.9b02496 Guohao Jin 1 , Xiaoling Wu 1 , Gang Cui 2 , Liqiang Liu 1 , Hua Kuang 1 , Chuanlai Xu 1
ACS Omega ( IF 3.7 ) Pub Date : 2020-07-13 , DOI: 10.1021/acsomega.9b02496 Guohao Jin 1 , Xiaoling Wu 1 , Gang Cui 2 , Liqiang Liu 1 , Hua Kuang 1 , Chuanlai Xu 1
Affiliation
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Diacetoxyscirpenol (DAS) is a highly toxic type A trichothecene, a natural contaminant in food and animal feed, which is a serious hazard to human and animal health. An anti-DAS mAb, 3H10, with high sensitivity and specificity, was prepared, and an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a lateral-flow immunochromatographic strip (ICA strip) were developed for rapid testing of DAS in rice samples. The 50% inhibitory concentration and limit of detection of ic-ELISA were 5.97 and 0.78 ng/mL, respectively. The recovery of rice samples ranged from 99.4 to 110.7%, demonstrating that the analytical method can be used to detect rice samples. The cutoff value of the lateral-flow ICA strip to DAS was 500 ng/g. The developed immunoassay method can provide an effective method of initially detecting and screening DAS in food and feed samples.
中文翻译：
开发用于检测大米中二乙酰氧基蕈酚的 ic-ELISA 和免疫层析试纸条测定法。
二乙酰氧基茑醇（DAS）是一种剧毒的A型单端孢菌烯，是食品和动物饲料中的天然污染物，对人类和动物健康造成严重危害。制备了高灵敏度和特异性的抗DAS单克隆抗体3H10，并开发了间接竞争酶联免疫吸附试验（ic-ELISA）和侧流免疫层析试纸条（ICA试纸条），用于快速检测DAS。大米样品。 ic-ELISA的50%抑制浓度和检测限分别为5.97和0.78 ng/mL。大米样品的回收率为99.4%~110.7%，表明该分析方法可用于大米样品的检测。侧向流动 ICA 条对 DAS 的截止值为 500 ng/g。所开发的免疫分析方法可以为食品和饲料样品中DAS的初步检测和筛选提供有效的方法。
更新日期：2020-07-28
中文翻译：
开发用于检测大米中二乙酰氧基蕈酚的 ic-ELISA 和免疫层析试纸条测定法。
二乙酰氧基茑醇（DAS）是一种剧毒的A型单端孢菌烯，是食品和动物饲料中的天然污染物，对人类和动物健康造成严重危害。制备了高灵敏度和特异性的抗DAS单克隆抗体3H10，并开发了间接竞争酶联免疫吸附试验（ic-ELISA）和侧流免疫层析试纸条（ICA试纸条），用于快速检测DAS。大米样品。 ic-ELISA的50%抑制浓度和检测限分别为5.97和0.78 ng/mL。大米样品的回收率为99.4%~110.7%，表明该分析方法可用于大米样品的检测。侧向流动 ICA 条对 DAS 的截止值为 500 ng/g。所开发的免疫分析方法可以为食品和饲料样品中DAS的初步检测和筛选提供有效的方法。
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