Spectroscopic single-molecule localization microscopy (sSMLM) simultaneously captures the spatial locations and full spectra of stochastically emitting fluorescent single molecules. It provides an optical platform to develop new multimolecular and functional imaging capabilities. While several open-source software suites provide subdiffraction localization of fluorescent molecules, software suites for spectroscopic analysis of sSMLM data remain unavailable. RainbowSTORM is an open-source ImageJ/FIJI plug-in for end-to-end spectroscopic analysis and visualization for sSMLM images. RainbowSTORM allows users to calibrate, preview and quantitatively analyze emission spectra acquired using different reported sSMLM system designs and fluorescent labels.

中文翻译：

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RainbowSTORM：一个开源ImageJ插件，用于光谱单分子定位显微镜（sSMLM）数据分析和图像重建。

光谱单分子定位显微镜（sSMLM）同时捕获随机发射的荧光单分子的空间位置和全光谱。它提供了开发新的多分子和功能成像功能的光学平台。尽管有几个开源软件套件提供了荧光分子的亚衍射定位，但是用于sSMLM数据的光谱分析的软件套件仍然不可用。RainbowSTORM是一个开源ImageJ / FIJI插件，用于sSMLM图像的端到端光谱分析和可视化。RainbowSTORM允许用户校准，预览和定量分析使用不同报告的sSMLM系统设计和荧光标记所获得的发射光谱。