Abstract

The aim of this study was to evaluate the toxicity of tucumã oil nanocapsules from the Amazon region in silver catfish, Rhamdia quelen. Fish were exposed to water treated with different concentrations of tucumã nanocapsules, white, solubilized oil and surfactant vehicles. After three days of exposure, fish were euthanized and liver, gills and brain removed for analysis of the dichlorofluorescein, nitric oxide and PicoGreen® assays. Plasma was collected for assay of hepatic transaminases. The nanocapsules had a diameter of 221 ± 1.27 nm, confirmed by atomic force microscopy. The oil nanocapsules were not toxic to this species of fish, but white nanocapsules and surfactant increased the levels of reactive oxygen species. Thus, nanocapsules are promising for the transport of tucumã oil. In view of the anti-inflammatory properties of this oil, it is possible to envisage its application in skin diseases for example, since they present essentially inflammatory conditions.

• Highlights

• The most abundant carotenoid in tucumã oil was all-trans-beta-carotene.

• Nanocapsules are good carriers for tucumã oil.

• Tucumã oil nanocapsules does nothas toxicity effect in catfish.

摘要

本研究的目的是评估来自亚马逊地区的 tucumã 油纳米胶囊对银鲶 ( Rhamdia quelen ) 的毒性. 鱼暴露在用不同浓度的 tucumã 纳米胶囊、白色、可溶性油和表面活性剂载体处理过的水中。暴露三天后，对鱼实施安乐死，取出肝脏、鳃和大脑，用于分析二氯荧光素、一氧化氮和 PicoGreen® 测定。收集血浆用于测定肝转氨酶。经原子力显微镜证实，纳米胶囊的直径为 221 ± 1.27 nm。油纳米胶囊对这种鱼没有毒性，但白色纳米胶囊和表面活性剂增加了活性氧的水平。因此，纳米胶囊有望用于 tucumã 油的运输。鉴于这种油的抗炎特性，可以设想它在例如皮肤病中的应用，因为它们基本上呈现炎症状态。

• 强调

• tucumã油中最丰富的类胡萝卜素是全-反式-β-胡萝卜素。

• 纳米胶囊是 tucumã 油的良好载体。

• Tucumã油纳米胶囊对鲶鱼没有毒性作用。