Abstract This study examined differential regulation of volatile terpenoids produced by rosemary (Rosmarinus officinalis L.) leaves in response to ionizing irradiation. Headspace volatile collection from rosemary leaves by solid-phase microextraction-gas chromatography-mass spectrometry analyses revealed that rosemary leaves biologically synthesized 26 species of volatile terpenoids and their relative ratio were changed after the exposure to γ-irradiation. Relative ratio of oxygenated monoterpenes such as borneol, (-)-verbenone, lemonol, and bornyl acetate (except for 1,8-cineol, linalool, chrysanthenone, camphor, and (+)-verbenone) and sesquiterpene hydrocarbons (i.e., caryophyllene-type) increased when the rosemary leaves were exposed to γ-ray in a dose-dependent manner. On the contrary, the change of the ratio of oxygenated sesquiterpenes such as caryophyllene oxide was negligible, and that of monoterpene hydrocarbons like α-pinene, camphene, myrcene, and limonene was down-regulated. These results indicate that γ-irradiation can modify chemical structures of volatile terpenes, and can, thus, be applied to reconstitute chemical constituents in aromatic plants.

中文翻译：

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迷迭香叶片中挥发性萜类成分对电离辐射响应的比较分析

摘要 本研究考察了迷迭香 (Rosmarinus officinalis L.) 叶子在响应电离辐射时产生的挥发性萜类化合物的差异调节。固相微萃取-气相色谱-质谱法对迷迭香叶顶空挥发物的收集表明，迷迭香叶生物合成了26种挥发性萜类化合物，它们的相对比例在γ-辐照后发生了变化。含氧单萜，如冰片、(-)-马鞭草酮、柠檬醇和醋酸冰片（1,8-桉树脑、芳樟醇、菊酮、樟脑和（+）-马鞭草酮除外）和倍半萜烃（即石竹烯-当迷迭香叶以剂量依赖性方式暴露于 γ 射线时，类型）增加。相反，氧化石竹烯等含氧倍半萜的比例变化可以忽略不计，α-蒎烯、莰烯、月桂烯和苎烯等单萜烃的比例变化被下调。这些结果表明，γ-辐照可以改变挥发性萜烯的化学结构，因此可用于重构芳香植物的化学成分。