A new protocol for long-term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers.,FEBS Open Bio

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A new protocol for long-term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers.
FEBS Open Bio ( IF 2.8 ) Pub Date : 2020-07-31 , DOI: 10.1002/2211-5463.12932
Biao Zhang _{1,

2} , Hai-Yang Wang _{1,

2} , Dong-Xing Wang _{1,

2} , Quan Zeng _{1,

2} , Zeng Fan _{1,

2} , Jia-Fei Xi _{1,

2} , Xue Nan _{1,

2} , Li-Juan He _{1,

2} , Jun-Nian Zhou _{1,

2,

3} , Xue-Tao Pei _{1,

2} , Wen Yue _{1,

2}

Affiliation

Liver cancer stem cells (L‐CSCs) are considered to be an important therapeutic target for hepatocellular carcinoma (HCC). This study provides a new in vitro long‐term culture model for a specific subpopulation of L‐CSCs enriched by cell surface markers. We combined CD13, CD133 and EpCAM to selectively enrich L‐CSCs, which we then cultured in modified chemically defined medium. The enriched L‐CSCs exhibited enhanced proliferation, self‐renewal and long‐term clonal maintenance ability as compared with non‐CSCs. Compared with wild‐type hepatocellular carcinoma, the expression of stemness surface markers, oncogenes, drug resistance and tumorigenicity in enriched L‐CSCs was significantly increased. In summary, the subpopulation of L‐CSCs still maintains cancer stem cell‐related phenotypes after 14 days of culture.

中文翻译：

长期培养通过细胞表面标志物富集的肝癌干细胞特定亚群的新方案。

肝癌干细胞（L-CSCs）被认为是肝细胞癌（HCC）的重要治疗靶点。本研究为富含细胞表面标志物的特定 L-CSCs 亚群提供了一种新的体外长期培养模型。我们将 CD13、CD133 和 EpCAM 结合起来选择性地富集 L-CSCs，然后将其在改良的化学成分确定的培养基中培养。与非 CSCs 相比，富集的 L-CSCs 表现出增强的增殖、自我更新和长期克隆维持能力。与野生型肝细胞癌相比，富集的 L-CSC 中干细胞表面标志物、癌基因、耐药性和致瘤性的表达显着增加。总之，L-CSCs 亚群在培养 14 天后仍保持与癌症干细胞相关的表型。

更新日期：2020-07-31

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