To investigate the characteristics of cytokines in patients with different HBV infection status and their correlation with HBV DNA, HBsAg, and HBeAg levels. Peripheral blood samples were collected from patients with chronic HBV infection in immune tolerance phase (IT), HBeAg‐positive chronic hepatitis B (CHB), and acute hepatitis B (AHB) groups, and levels of cytokines were detected by Luminex technique, and analyzed by FLEXMAP 3D analyzer. The correlation between cytokines and HBV DNA load, HBsAg, HBeAg, and alanine aminotransferase (ALT) level in patients with chronic HBV infection was analyzed. In total 312 subjects (184 males and 128 females) were enrolled in the study. There were significant differences among IT, CHB, and AHB groups in Flt‐3L value (P = .003; H = 12.312), IFN‐γ (P = .001; H = 11.723), IL‐10 (P = .001; H = 18.736), IL‐17A ((P = .001; H = 12.735), and TGF‐β1 (P = .001; Z = 48.571). IFN‐α2 levels in CHB group were significantly higher than those in IT and AHB groups (15.24 vs 35.78 pg/mL, P = .000; Z = 3.727; 13.88 vs 35.78 pg/mL, P = .024; Z = −2.258. In CHB group, the levels of HBsAg and ALT were positively correlated with the levels of IL‐10 (r = .173; P = .006; r = 0.176; P = .006, respectively), while HBeAg level was positively correlated with the IFN‐α2 level (r = .153; P = .016). In AHB group, the HBsAg level was positively correlated with Flt‐3L, IFN‐α2, IL‐10, and IL‐6 (r = .402; P = .023; r = .436; P = .016; r = .524, P = .002; r = .405; P = .022, respectively). HBeAg level was positively correlated with IFN‐γ and IL‐17A levels (r = .400; P = .023; r = .373; P = .036, respectively), and ALT level was positively correlated with IL‐6 levels (r = .367; P = .039). In either AHB or CHB group, HBV DNA load was only related to TGF‐β level (r = .493; P = .004; r = −.218, P = 0.009 respectively). The correlation between Flt‐3L and HBsAg (F = 7.422; P = .007); IL‐17, IL‐6, and HBeAg (F = 5.757; P = .017; F = 6.156; P = .014) were statistically significant. There was significant correlation between TGF‐β2 and HBV DNA (F = 11.795; P = .001), and between ALT and HBsAg, HBV DNA (F = 26.089; P = .000; F = 4.724; P = .031). HBsAg, HBeAg, and HBV DNA were correlated with cytokines and ALT in patients with HBV infection. The level of IFN‐α2 was significantly higher in patients with CHB. HBV DNA load was only correlated with the level of TGF‐β in acute or CHB.

中文翻译：

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细胞因子与乙型肝炎病毒及其抗原的关联。


探讨不同HBV感染状态患者细胞因子的特征及其与HBV DNA、HBsAg、HBeAg水平的相关性。采集慢性乙型肝炎病毒感染免疫耐受期（IT）组、H​​BeAg阳性慢性乙型肝炎（CHB）组和急性乙型肝炎（AHB）组患者的外周血样本，采用Luminex技术检测细胞因子水平，并进行分析。通过 FLEXMAP 3D 分析仪。分析慢性乙型肝炎病毒感染患者细胞因子与HBV DNA载量、HBsAg、HBeAg、丙氨酸转氨酶（ALT）水平的相关性。共有 312 名受试者（184 名男性和 128 名女性）参加了该研究。 IT、CHB 和 AHB 组之间 Flt-3L 值 ( P = .003; H = 12.312)、IFN-γ ( P = .001; H = 11.723)、IL-10 ( P = .001) 存在显着差异。 ; H = 18.736)、IL-17A (( P = .001; H = 12.735) 和 TGF-β1 ( P = .001; Z = 48.571)。CHB 组的 IFN-α2 水平显着高于 IT 组和 AHB 组（15.24 vs 35.78 pg/mL， P = .000； Z = 3.727；13.88 vs 35.78 pg/mL， P = .024； Z = -2.258。在 CHB 组中，HBsAg 和 ALT 水平呈正相关与 IL-10 水平呈正相关（分别为r = .173； P = .006； r = 0.176； P = .006），而 HBeAg 水平与 IFN-α2 水平呈正相关（ r = .153； P =在 AHB 组中，HBsAg 水平与 Flt-3L、IFN-α2、IL-10 和 IL-6 呈正相关（ r = .402； P = .023； r = .436； P = . 016； r = .524， P = .002； r = .405； P = .022，分别）。 HBeAg 水平与 IFN-γ 和 IL-17A 水平呈正相关（分别为r = .400； P = .023； r = .373； P = .036），并且 ALT 水平与 IL-6 水平呈正相关（ r = .367； P = .039)。在 AHB 或 CHB 组中，HBV DNA 载量仅与 TGF-β 水平相关（分别为r = .493； P = .004； r = -.218， P = 0.009）。 Flt-3L 与 HBsAg 之间的相关性（ F = 7.422； P = .007）； IL-17、IL-6 和 HBeAg ( F = 5.757； P = .017； F = 6.156； P = .014) 具有统计学意义。 TGF-β2 与 HBV DNA 之间存在显着相关性 ( F = 11.795; P = .001)，而 ALT 与 HBsAg、HBV DNA 之间存在显着相关性 ( F = 26.089; P = .000; F = 4.724; P = .031)。 HBV 感染患者的 HBsAg、HBeAg 和 HBV DNA 与细胞因子和 ALT 相关。慢性乙型肝炎患者的 IFN-α2 水平显着升高。 HBV DNA 载量仅与急性或慢性乙型肝炎中的 TGF-β 水平相关。