Performance of Bt-susceptible and -heterozygous dual-gene resistant genotypes of Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) in seed blends of non-Bt and pyramided Bt maize,Insect Science

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Performance of Bt-susceptible and -heterozygous dual-gene resistant genotypes of Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) in seed blends of non-Bt and pyramided Bt maize
Insect Science ( IF 2.9 ) Pub Date : 2020-07-14 , DOI: 10.1111/1744-7917.12850
Marcelo Dimase ₁ , Sebe Brown ₂ , Graham P Head ₃ , Paula A Price ₃ , Wade Walker ₄ , Wenbo Yu ₁ , Fangneng Huang ₁

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A seed blend refuge has been implemented in the U.S. Corn Belt for Bt maize resistance management. The fall armyworm, Spodoptera frugiperda (J.E. Smith), is a target pest of Bt maize in the Americas. The larvae of this pest are mobile, which may affect the efficacy of seed blend refuges. In this study, field and greenhouse trials were conducted to determine the performance of Bt-susceptible (aabb) and -heterozygous dual-gene-resistant (AaBb) genotypes of S. frugiperda in seed blends of non-Bt and pyramided Bt maize. Three field trials evaluated larval survival, larval growth, and plant injury with aabb in seed blends of Bt maize expressing Cry1A.105/Cry2Ab2/Vip3A with 0–30% non-Bt seeds. Greenhouse tests investigated the performance of aabb and AaBb in seed blends of Cry1A.105/Cry2Ab2 with 0–30% non-Bt seeds. In pure non-Bt maize plots, after 9–13 d of neonates being released on the plants, 0.39 and 0.65 larvae/plant survived with leaf injury ratings of 4.7 and 5.9 (Davis's 1–9 scale) in the field and greenhouse, respectively. In contrast, live larvae and plant injury were virtually not observed on Bt plants across all planting patterns. Larval occurrence and plant injury by aabb on non-Bt plants were similar between seed blends and pure non-Bt plantings, suggesting that the blended refuges could provide an equivalent susceptible population as structured refuge under the test conditions. In the greenhouse, the two insect genotypes in seed blends performed similarly, indicating that the seed blends did not provide more favorable conditions for AaBb over aabb. The information generated from this study should be useful in managing S. frugiperda and evaluating if send blends could be suitable refuge options for Bt resistance management in the regions where the insect is a primary target pest.

中文翻译：

草地夜蛾（JE Smith）（鳞翅目：夜蛾科）的 Bt 易感和杂合双基因抗性基因型在非 Bt 和锥状 Bt 玉米的种子混合物中的表现

美国玉米带已实施种子混合避难所，用于 Bt 玉米抗性管理。秋季粘虫Spodoptera frugiperda (JE Smith) 是美洲 Bt 玉米的目标害虫。这种害虫的幼虫是移动的，这可能会影响种子混合避难所的功效。在这项研究中，进行了田间和温室试验以确定S. frugiperda的 Bt 易感 (aabb) 和杂合双基因抗性 (AaBb) 基因型的性能在非 Bt 和金字塔型 Bt 玉米的种子混合物中。三项田间试验用 aabb 在表达 Cry1A.105/Cry2Ab2/Vip3A 的 Bt 玉米与 0-30% 非 Bt 种子的种子混合物中评估了幼虫存活、幼虫生长和植物损伤。温室试验研究了 aabb 和 AaBb 在 Cry1A.105/Cry2Ab2 与 0-30% 非 Bt 种子的种子混合物中的性能。在纯非 Bt 玉米地块中，在将幼虫释放到植物上 9-13 天后，0.39 和 0.65 只幼虫/植物在田间和温室中存活，叶片损伤等级分别为 4.7 和 5.9（戴维斯的 1-9 级） . 相比之下，在所有种植模式中，几乎没有在 Bt 植物上观察到活幼虫和植物损伤。aabb 对非 Bt 植物的幼虫发生和植物损伤在混合种子和纯非 Bt 植物之间相似，表明混合避难所可以在测试条件下提供与结构化避难所等效的易感人群。在温室中，种子混合物中的两种昆虫基因型表现相似，表明种子混合物没有为 AaBb 提供比 aabb 更有利的条件。从这项研究中产生的信息应该有助于管理S. frugiperda并评估发送混合物是否可以作为在昆虫是主要目标害虫的地区进行 Bt 抗性管理的合适避难选择。

更新日期：2020-07-14

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