Background

Ankylosing spondylitis (AS) is a chronic inflammatory disease, whose pathogenesis is still unclear. Many studies show the proteins in extracellular vesicle (EVs) would change regularly in many diseases. The study aims to explore the proteins contents of serum-derived EVs in AS patients.

Methods

EVs were separated by ExoQuick^TM kit. The protein profiles of AS patients and healthy subjects were analyzed by Label-free-liquid chromatography mass spectrometry (LC-MS/MS) technology. Enzyme-linked immunosorbent assay (ELISA) was used to verify the levels of the differently expressed proteins. Receiver operation characteristic (ROC) curves and bioinformatic analysis were conducted.

Results

Six hundred and ten serum-derived EVs proteins from AS patients were detected. Seventy-three diferentially expressed proteins were found in AS group, compared with healthy subjects. Of these, 31 proteins were up-regulated in AS group, while 42 proteins were down-regulated. ELISA result showed that EVs-derived serum amyloid A-1 (SAA1) was higher in AS group, which was consistent with the Label-free-LC-MS/MS data. ROC curves result revealed that the area under curve (AUC) value of EVs-derived SAA1 for AS was 0.768 (0.652–0.885). Bioinformatic analysis revealed that the differently expressed proteins in AS group were significantly involved in “complement and coagulation cascades”, “staphylococcus aureus infection”, “systemic lupus erythematosus” and “PI3K-Akt signaling pathway”.

Conclusions

The protein profiles of serum-derived EVs in AS patients and healthy subjects were different. EVs-derived SAA1 may be a potential biomarkes of AS. The function analysis indicated that the differentially expressed proteins may potentially participate in immune response.

中文翻译：

---

强直性脊柱炎患者血清来源的细胞外囊泡的蛋白质组学分析。

背景

强直性脊柱炎（AS）是一种慢性炎症性疾病，其发病机理仍不清楚。许多研究表明，在许多疾病中，细胞外囊泡（EVs）中的蛋白质会定期变化。这项研究旨在探讨AS患者血清来源的EV的蛋白质含量。

方法

电动汽车通过ExoQuick ^TM套件分离。通过无标记液相色谱质谱（LC-MS / MS）技术分析AS患者和健康受试者的蛋白质谱。酶联免疫吸附测定（ELISA）用于验证不同表达的蛋白质的水平。进行了接收者操作特征（ROC）曲线和生物信息学分析。

结果

检测到来自AS患者的610种血清来源的EVs蛋白。与健康受试者相比，在AS组中发现了73种差异表达的蛋白质。其中，AS组中31种蛋白被上调，而42组蛋白被下调。ELISA结果表明，AS组的EVs血清淀粉样蛋白A-1（SAA1）较高，这与无标记LC-MS / MS数据一致。ROC曲线结果显示，EV衍生的SAA1对AS的曲线下面积（AUC）值为0.768（0.652-0.885）。生物信息学分析显示，AS组中差异表达的蛋白显着参与了“补体和凝血级联”，“金黄色葡萄球菌感染”，“系统性红斑狼疮”和“ PI3K-Akt信号通路”。

结论

AS患者和健康受试者血清来源的EV的蛋白质谱不同。EV衍生的SAA1可能是AS的潜在生物标记。功能分析表明差异表达的蛋白质可能潜在参与免疫反应。