Abstract Polyphenols found in fruits and vegetables are of great interest due to their health benefits. Gallic acid (GA) is an antioxidant with cytotoxic effects. Microspheres are drug carrier systems, where the active substance is trapped in matrix. A controlled release profile is aimed at the target region by preserving the loss of substance and activity. In this study, GA containing microspheres were formed with sodium alginate (NaAlg) in formulations with different ratios. The arrest efficiencies ranged between 11.26 and 72.64%. Release studies were performed at pH 7.4. Optimum conditions were determined as GA/NaAlg ratio 1/8. The microspheres were found to arrest GA and exhibit a controlled release profile. Microspheres were characterized by scanning electron microscope (SEM), differential scanning calorimetry (DSC), X-Ray diffraction (XRD), Fourier transform infrared spectrometer (FTIR) analysis. GA retained its antioxidant activity determined by DPPH (2,2-diphenyl-1-picrylhydrazyl) method and anticancer activity on Caco-2 cells. Further studies are necessary on GA-loaded NaAlg microspheres which have high potential to be used in adjunct and complementary therapies.

中文翻译：

---

没食子酸负载海藻酸钠微球对结肠癌的抗氧化和抗癌活性

摘要 在水果和蔬菜中发现的多酚因其健康益处而备受关注。没食子酸 (GA) 是一种具有细胞毒性作用的抗氧化剂。微球是药物载体系统，其中活性物质被困在基质中。控释曲线旨在通过保留物质和活性的损失来针对目标区域。在这项研究中，含有 GA 的微球与海藻酸钠 (NaAlg) 以不同比例的配方形成。逮捕效率介于 11.26 和 72.64% 之间。在 pH 7.4 下进行释放研究。最佳条件确定为 GA/NaAlg 比率 1/8。发现微球可以阻止 GA 并表现出控释特性。微球通过扫描电子显微镜（SEM）、差示扫描量热法（DSC）、X射线衍射（XRD）、傅里叶变换红外光谱仪 (FTIR) 分析。GA 保留了由 DPPH（2,2-diphenyl-1-picrylhydrazyl）法测定的抗氧化活性和对 Caco-2 细胞的抗癌活性。需要对负载 GA 的 NaAlg 微球进行进一步研究，这些微球具有用于辅助和补充疗法的巨大潜力。