Lactic acid bioconversion processes have numerous advantages over the chemical synthesis route, not only due to the high-titer yield of the final product with great optical purity, but also due to the possibility of utilizing lignocellulosic biomass feedstocks as carbon source in an economic and environmentally friendly way. In the present study, beechwood and pine were pretreated with a novel mild oxidative organosolv process to produce cellulose-rich solid fractions, which were tested for their ability to support the growth and high lactic acid productivity of Lactobacillus delbrueckii subsp. bulgaricus (ATCC® 11842). We employed a simultaneous saccharification and fermentation (SSF) strategy in batch cultures with 9% w v⁻¹ solids loading. The results for beechwood showed the highest production of 62 g L⁻¹ lactic acid after 72 h of incubation, corresponding to a yield of 0.69 g g⁻¹ of biomass (82.7% of the theoretical maximum yield) and a productivity of 0.86 g L⁻¹ h⁻¹. In the case of pine, the productivity was lower at 0.51 g L⁻¹ h⁻¹, leading to accumulation of 36.4 g L⁻¹ lactic acid, corresponding to a yield of 0.40 g g⁻¹ of biomass (41.4% of the theoretical maximum yield). Our study suggests that L. delbrueckii subsp. bulgaricus is an efficient lactic acid bacterial strain for the production of optically pure d-lactic acid from non-edible, organosolv pretreated hardwood and softwood biomass for the synthesis of bio-based plastics and other products.

乳酸生物转化工艺在化学合成路线上具有众多优势，这不仅是因为最终产品的滴定度高，光学纯度高，而且还因为在经济和环境方面可以利用木质纤维素生物质原料作为碳源友好的方式。在本研究中，用一种新颖的温和氧化有机溶剂工艺对山毛榉木和松木进行预处理，以生产富含纤维素的固体级分，并测试了它们支持德氏乳杆菌亚种的生长和高乳酸生产率的能力。保加利亚（ATCC®11842）。我们在9％wv ^-1的分批培养中采用了同时糖化和发酵（SSF）策略固体装载。对于山毛榉结果显示出最高的生产62克L- ^-1乳酸孵育72小时后，对应于0.69克g的收率^-1生物质（理论最大收率的82.7％）和0.86克L-生产率的^{- 1} 小时^-1。在松树的情况下，生产率较低，为0.51 g L ^-1  h ^-1，导致积累了36.4 g L ^-1乳酸，对应于0.40 g g ^-1的生物质产量（理论最大值的41.4％）让）。我们的研究表明，L。delbrueckii亚种。保加利亚是一种高效的乳酸菌菌株，用于从不可食用的经过有机溶剂预处理的硬木和软木生物质生产光学纯的d-乳酸，用于合成生物基塑料和其他产品。