This study aimed to evaluate the aflatoxin B₁ (AFB₁) adsorption capacity of the seaweed Lithothamnium calcareum in vitro and to prevent aflatoxicosis in broiler chickens. In vitro adsorption assays were performed at a single AFB₁ concentration (1 μg/mL) and four seaweed concentrations (0.50, 1, 1.5 and 2 mg/mL) at pH 3 and pH 6. The maximum adsorption was obtained at the lowest seaweed content (0.62 and 0.78 μg/mg). Male broiler chickens (256) were housed in metallic cages. Experimental diets were T1 (control), 18 μg/kg AFB₁; T2, 18 μg/kg AFB₁ and 0.2% L. calcareum (2.0 kg/ton); T3, 1018 μg/kg AFB₁; and T4, 1018 μg/kg AFB₁ and 0.2% L. calcareum. Performance parameters (live weight, weight gain and feed conversion rate) improved when seaweed was applied. The aspartate-aminotransferase and alanine-aminotransferase levels tend to decrease in birds receiving only seaweed, also the uric acid levels reduced significantly (P ˂ 0.05), while birds receiving only AFB₁ increased the biochemical parameter levels. The livers from animals fed with AFB₁ showed histopathological alterations with disorganization of periportal hepatocytes, necrosis with multifocal coagulation and mild fat degeneration; the livers from T4 had normal appearance. Lithothamnium calcareum was able to prevent aflatoxicosis in broiler chickens and also improved their zootechnical performance.

这项研究旨在评估海藻钙碎石在体外的黄曲霉毒素B ₁（AFB ₁）吸附能力，并预防肉鸡的黄曲霉毒素中毒。在pH 3和pH 6下，以单一AFB ₁浓度（1μg/ mL）和四种海藻浓度（0.50、1、1.5和2 mg / mL）进行体外吸附测定。最大的吸附在最低的海藻上获得含量（0.62和0.78μg/ mg）。将雄性肉鸡（256）放在金属笼中。实验饮食为T1（对照），18μg/ kg AFB ₁；T2，18μg/ kg AFB ₁和0.2％钙乳杆菌（2.0 kg /吨）; T3，1018μg/ kg AFB ₁ ; 和T4，1018μg/ kg AFB₁和0.2％钙乳杆菌。使用海藻后，性能参数（活体重，增重和饲料转化率）得到改善。天冬氨酸转氨酶和丙氨酸氨基转移酶水平往往在接收鸟类降低仅海藻，也尿酸水平显著降低（P  ˂0.05），而鸟类仅接收AFB ₁增加的生化参数的水平。饲喂AFB _1的动物的肝脏表现出组织病理学改变，包括门静脉周围的肝细胞紊乱，多灶性凝结坏死和轻度脂肪变性。T4的肝脏外观正常。石灰石 能够预防肉鸡的黄曲霉毒素中毒，还改善了它们的动物技术性能。