We report the high production of stilbenes, including resveratrol and viniferin, in grapevine (Vitis labruscana L.) cell cultures through elicitation with methyl jasmonate (MeJA) and stevioside (STE). Methyl-β-cyclodextrin (MeβCD) is widely used as a solubilizer for resveratrol production. For the first time, we used STE as a solubilizer for stilbene production in plant cell cultures. MeJA was most effective elicitor in activating VvSTS expression and stimulating stilbene biosynthesis in grapevine cell cultures. The maximum concentration of δ-viniferin (892.2 mg/L) production with a small amount of trans-resveratrol (12.2 mg/L) was observed in the culture medium by co-treatment of cells with MeJA and STE, whereas the highest level of trans-resveratrol (371.9 mg/L) with a slight amount of δ-viniferin (11.5 mg/L) was accumulated in the culture medium of cells treated with MeJA and MeβCD. However, neither trans-resveratrol nor δ-viniferin were significantly elevated within the cells by the applications. Notably, predominant production of δ-viniferin and trans-resveratrol was observed in shake and static flask culture medium, respectively, by co-treatment of MeJA and STE. Furthermore, stilbene compounds of resveratrol, ε-viniferin, and δ-viniferin were mainly produced in a 3-L bioreactor culture following elicitation of cells with MeJA and STE. These results provide new strategies for conditional, high-level production of resveratrol and viniferin in cell cultures by utilizing the solubilizing properties of STE or MeβCD.

中文翻译：

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茉莉酸甲酯和甜菊糖苷的诱导在葡萄细胞培养基中诱导斯蒂苯类的细胞外产生

我们报告了通过与茉莉酸甲酯（MeJA）和甜菊糖苷（STE）的诱变，葡萄树（Vitis labruscana L.）细胞培养物中的丁苯橡胶，包括白藜芦醇和viniferin的高产量。甲基-β-环糊精（MeβCD）被广泛用作白藜芦醇生产的增溶剂。我们首次使用STE作为增溶剂在植物细胞培养物中生产二苯乙烯。MeJA是激活VvSTS表达和刺激葡萄细胞培养物中二苯乙烯合成的最有效引发剂。通过与MeJA和STE共同处理细胞，在培养基中观察到最大量的δ-葡萄素（892.2 mg / L）和少量的反式白藜芦醇（12.2 mg / L）产生，而最高水平的反式在用MeJA和MeβCD处理过的细胞的培养基中积累了白藜芦醇（371.9 mg / L）和少量的δ-viniferin（11.5 mg / L）。然而，通过该应用，在细胞内反式白藜芦醇和δ-viniferin都没有显着升高。值得注意的是，通过共同处理MeJA和STE，在摇瓶和静态烧瓶培养基中分别观察到主要产生δ-葡萄素和反式白藜芦醇。此外，在用MeJA和STE诱导细胞后，在3-L生物反应器培养物中主要产生白藜芦醇，ε-viniferin和δ-viniferin的二苯乙烯化合物。这些结果为利用STE或MeβCD的增溶特性在细胞培养中有条件地高水平生产白藜芦醇和viniferin提供了新的策略。