To improve nicotinic acid (NA) yield and meet industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase, inorganic materials were added to the SA-PVA immobilized cells to improve mechanical strength and mass transfer performance. The concentrations of inorganic materials were optimized to be 2.0% silica and 0.6% CaCO₃. The optimal pH and temperature for SA-PVA immobilized cells and composite immobilized cells were both 8.0 and 45 °C, respectively. The half-lives of composite immobilized cells were 271.48, 150.92, 92.92 and 33.12 h, which were 1.40-, 1.35-, 1.22- and 1.63-fold compared to SA-PVA immobilized cells, respectively. The storage stability of the composite immobilized cells was slightly increased. The composite immobilized cells could convert 14 batches of 3-cyanopyridine with feeding concentration of 250 mM and accumulate 418 g ·L⁻¹ nicotinic acid, while the SA-PVA immobilized cells accumulated 346 g L⁻¹ nicotinic acid.

为提高海藻酸钠-聚乙烯醇(SA-PVA)固定化含有腈水解酶的恶臭假单胞菌mut-D3细胞的烟酸(NA)产率和工业应用需求，在SA-PVA固定化细胞中加入无机材料以提高机械强度和传质性能。无机材料的浓度优化为 2.0% 二氧化硅和 0.6% CaCO ₃. SA-PVA 固定化细胞和复合固定化细胞的最佳 pH 值和温度分别为 8.0 和 45°C。复合固定化细胞的半衰期分别为 271.48、150.92、92.92 和 33.12 小时，分别是 SA-PVA 固定化细胞的 1.40、1.35、1.22 和 1.63 倍。复合固定化细胞的储存稳定性略有提高。复合固定化细胞可以转化14批3-氰基吡啶，进料浓度为250 mM，积累418 g·L ^-1烟酸，而SA-PVA固定化细胞积累346 g L ^-1烟酸。