A sensitive fluorometric sensor for Ag+ based on the hybridization chain reaction coupled with a glucose oxidase dual-signal amplification strategy,RSC Advances

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A sensitive fluorometric sensor for Ag+ based on the hybridization chain reaction coupled with a glucose oxidase dual-signal amplification strategy
RSC Advances ( IF 3.9 ) Pub Date : 2020-7-13 , DOI: 10.1039/d0ra04202a
Yubin Li ₁ , Ling Xie ₁ , Jiaming Yuan ₁ , Huazhong Liu ₁

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In this work, an efficient and sensitive fluorometric sensor was developed to detect silver ions (Ag⁺). It is based on the cytosine–Ag⁺–cytosine (C–Ag⁺–C) structure via a dual-signal amplification strategy using glucose oxidase (GOx) and the hybridization chain reaction (HCR). A silver-coated glass slide (SCGS) acts as an ideal material for separation. Cytosine rich (C-rich) capture DNA (C-DNA) assembled themselves on the SCGS via Ag–S bonds and hybridized with signal DNA (S-DNA) to trigger the HCR. With specific base-pairing, the S-DNA and HCR products bind on the SCGS. Then, the GOx–biotin–streptavidin (SA) complexes bind to the HCR products through SA–biotin interactions. Owing to the formation of a particular C–Ag⁺–C structure between two neighboring C-rich C-DNA on the SCGS, the C-DNA/S-DNA/HP1-GOx/HP2-GOx complex gradually moved away from the SCGS as the concentration of Ag⁺ increased and the combined GOx fell into the buffer. H₂O₂ could be generated during the oxidation of glucose, catalyzed by GOx in the buffer. Afterward, H₂O₂ could oxidize the substrate (3-(p-hydroxyphenyl)-propanoic acid) when Horseradish peroxidase was present, giving rise to blue fluorescence. The proposed strategy reached a limit of detection (LOD) of 1.8 pmol L⁻¹ with a linear detection range of 5 to 1000 pmol L⁻¹ for Ag⁺. Moreover, this assay has been commendably used for the detection of Ag⁺ in actual samples with fairly good results.

中文翻译：

基于杂交链反应结合葡萄糖氧化酶双信号放大策略的Ag+灵敏荧光传感器

在这项工作中，开发了一种高效灵敏的荧光传感器来检测银离子（Ag ⁺）。它基于胞嘧啶-Ag ⁺ -胞嘧啶(C-Ag ⁺ -C) 结构，通过使用葡萄糖氧化酶 (GOx) 和杂交链式反应 (HCR) 的双信号放大策略。镀银载玻片 (SCGS) 是分离的理想材料。富含胞嘧啶 (C-rich) 的捕获 DNA (C-DNA) 通过自身组装在 SCGS上Ag-S 键并与信号 DNA (S-DNA) 杂交以触发 HCR。通过特定的碱基配对，S-DNA 和 HCR 产物结合在 SCGS 上。然后，GOx-生物素-链霉亲和素 (SA) 复合物通过 SA-生物素相互作用与 HCR 产物结合。由于SCGS上两个相邻的富含C的C-DNA之间形成了特定的C-Ag ⁺ -C结构，C-DNA/S-DNA/HP1-GOx/HP2-GOx复合物逐渐远离SCGS随着 Ag ⁺浓度的增加和组合的 GOx 落入缓冲液中。H ₂ O ₂可在葡萄糖氧化过程中产生，由缓冲液中的 GOx 催化。之后，H ₂ O ₂可以氧化底物（3-( p-羟基苯基)-丙酸)，当存在辣根过氧化物酶时，会产生蓝色荧光。所提出的策略达到了 1.8 pmol L ^-1的检测限 (LOD)， Ag ⁺的线性检测范围为 5 至 1000 pmol L ^-1。^{此外，该方法在实际样品中Ag +}的检测也取得了不错的效果，值得称道。

更新日期：2020-07-13

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