Systematic Screening and Deep Analysis of CoPt Binding Peptides Leads to Enhanced CoPt Nanoparticles Using Designed Peptides.,Bioconjugate Chemistry

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Systematic Screening and Deep Analysis of CoPt Binding Peptides Leads to Enhanced CoPt Nanoparticles Using Designed Peptides.
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2020-07-13 , DOI: 10.1021/acs.bioconjchem.0c00348
Rosie M Jarrald ₁ , Aw W Liang Alvin ₂ , Andrea E Rawlings ₁ , Masayoshi Tanaka ₂ , Mina Okochi ₂ , Sarah S Staniland ₁

Affiliation

Using protein and peptide additives to direct the crystallization of inorganic materials is a very attractive and environmentally friendly strategy to access complex and sometimes inaccessible mineral phases. CoPt is a very desirable high-magnetoanisotropic material in its L1₀ phase, but this is acquired by annealing at high temperatures which is incompatible with delicate nanomaterial assembly. Previous studies identified one peptide with high affinity to CoPt and four peptides with high affinity to FePt L1₀ phase nanoparticles (NPs) through phage display biopanning selection. While synthesis mediated by these peptides offered a small degree of L1₀ character to the NPs, they do not have the magnetoanistropy required for applications. In this study, we improve the activity of peptide directed crystallization by designing second generation peptides. We use the five literature sequences (LS) to probe the binding affinity deeper through dissection (alanine scanning), reduction (truncations), and substitution of the LS to find key amino acids and motifs. This is performed using a SPOT peptide array, importantly probing interactions at three stages of NP formation: with precursor, during synthesis, and with NPs. We found four universal features: 1) the importance of basic residues, particularly lysine flanking both ends of the sequence; 2) the importance of methionine; 3) shorter sequences show higher affinity than longer ones; and 4) acidic residues have a negative impact on binding with aspartic acid less favorable than glutamic acid. However, an acidic amino acid benefits, presumably to balance charge. The short motif KSLS had high affinity in all assays. Three sequences were selected from the screening, and three sequences were designed from the rules above. These were used to mediate a green synthesis of CoPt nanoparticles. The screened peptides mediated the formation of NPs with improved coercivity (90–110 Oe) compared to the LS (30–80 Oe), while the designed peptides facilitated formation of CoPt NPs with the highest coercivity (109 to 132 Oe), representing a massive improvement on L1₀ character. This result along with deeper insight this methodology brings offers vast potential for the future.

中文翻译：

CoPt结合肽的系统筛选和深入分析导致使用设计的肽增强的CoPt纳米颗粒。

使用蛋白质和多肽添加剂来引导无机材料的结晶是一种非常有吸引力且对环境友好的策略，可用于获取复杂的，有时是不可访问的矿物相。CoPt是一种非常理想的处于L1 ₀相的高磁各向异性材料，但这是通过在高温下退火而获得的，这与精密的纳米材料组装不兼容。先前的研究通过噬菌体展示生物淘选筛选确定了一种对CoPt具有高亲和力的肽和四种对FePt L1 ₀相纳米颗粒（NPs）具有高亲和力的肽。尽管这些肽介导的合成提供了较小的L1 ₀NP的特性，它们不具有应用所需的磁各向异性。在这项研究中，我们通过设计第二代肽提高了肽定向结晶的活性。我们使用五个文献序列（LS），通过解剖（丙氨酸扫描），还原（截短）和LS取代来更深入地探索结合亲和力，以找到关键的氨基酸和基序。这是使用SPOT肽阵列进行的，重要的是探测NP形成的三个阶段的相互作用：与前体，合成期间以及与NP的相互作用。我们发现了四个普遍特征：1）基本残基的重要性，特别是位于序列两端的赖氨酸。2）蛋氨酸的重要性；3）较短的序列比较长的序列显示更高的亲和力；4）酸性残基对与天冬氨酸的结合产生不利影响，但不如谷氨酸好。但是，酸性氨基酸有利于平衡电荷。短基序KSLS在所有测定中均具有高亲和力。从筛选中选择了三个序列，并且根据以上规则设计了三个序列。这些被用来介导CoPt纳米颗粒的绿色合成。与LS（30–80 Oe）相比，筛选的肽介导了具有更高矫顽力（90–110 Oe）的NP的形成，而设计的肽则促进了具有最高矫顽力（109至132 Oe）的CoPt NP的形成。 L1的巨大改进从筛选中选择了三个序列，并且根据以上规则设计了三个序列。这些被用于介导CoPt纳米颗粒的绿色合成。与LS（30–80 Oe）相比，筛选的肽介导了具有更高矫顽力（90–110 Oe）的NP的形成，而设计的肽则促进了具有最高矫顽力（109至132 Oe）的CoPt NP的形成。 L1的巨大改进从筛选中选择了三个序列，并且根据以上规则设计了三个序列。这些被用来介导CoPt纳米颗粒的绿色合成。与LS（30–80 Oe）相比，筛选的肽介导了具有更高矫顽力（90–110 Oe）的NP的形成，而设计的肽则促进了具有最高矫顽力（109至132 Oe）的CoPt NP的形成。 L1的巨大改进_0个字符。这一结果以及这种方法带来的更深刻见解为未来提供了巨大的潜力。

更新日期：2020-08-19

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