Purpose

Several past studies using a mouse model of radiation-induced AML (rAML) have shown that hemizygous deletion of the Sfpi1 gene (HDSG) is an initiating event for the development of rAML. In this study, we examined the difference in frequency of HDSG in hematopoietic stem cells (HSCs) Rich hematopoietic Cell population (HRCs) from bone marrow (BM) and spleen of C3H mice irradiated with 3 Gy X-rays.

Materials and methods

8-weeks old male C3H mice were irradiated 3Gy of whole body X-ray (1 Gy/min) and mice were sacrificed at 1, 4, 8, and 26 weeks. Then, HSPCs were isolated from BM of femur and spleen, the frequency of HRCs with Sfpi1 gene deletion was analyzed by fluorescence in situ hybridization (FISH).

Results and conclusions

The frequency of HRCs with HDSG in both BM and spleen was increased 1 week after X-irradiation. Then, the frequency of HRCs with HDSG in BM showed a gradual decrease from 4 to 26 weeks, whereas HRCs with HDSG in spleen remained high, even at 26 weeks after X-irradiation. HDSG is less likely to be eliminated, particularly in the spleen, after X-irradiation. The spleen as well as BM of the femur may be major sites of rAML development.

中文翻译：

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具有Sfpi1缺失的造血细胞的细胞动力学在X射线照射的小鼠的骨髓和脾脏中以不同的频率出现。

目的

过去使用辐射诱导的AML（rAML）小鼠模型进行的几项研究表明，Sfpi1基因（HDSG）的半合子缺失是rAML发生的起始事件。在这项研究中，我们检查了用3 Gy X射线照射的C3H小鼠的骨髓（BM）和脾脏中造血干细胞（HSC），富血造血细胞群体（HRC）中HDSG的频率差异。

材料和方法

对8周大的雄性C3H小鼠进行3Gy的全身X射线照射（1 Gy / min），并在第1、4、8和26周处死小鼠。然后，从股骨和脾的BM中分离出HSPC，通过荧光原位杂交（FISH）分析具有Sfpi1基因缺失的HRC的频率。

结果与结论

X射线照射1周后，BM和脾脏中带有HDSG的HRC的频率增加。然后，BM中带有HDSG的HRC的频率从4周逐渐减少到26周，而脾脏中带有HDSG的HRC甚至在X射线照射后的26周仍然很高。X射线照射后，HDSG消除的可能性较小，尤其是在脾脏中。股骨的脾脏和BM可能是rAML发育的主要部位。