ABSTRACT Successful reproduction in angiosperms is dependent on the highly synchronous development of their male and female gametophytes and the ensuing fusion of the gametes from these reproductive tissue types. When crossing a T-DNA insertion line sdk1-7-/-(Salk_024564), one of the S-domain receptor kinases involved in ABA responses with a fast neutron deletion line abi3-6-/-, the F1 heterozygotes (sdk1-7+/-abi3-6 +/-) displayed 50% ovule abortion suggesting a likely gametophytic defects. We identified and characterized an early stage female gametophyte developmental defect in the heterozygous mutant ovules. Recombination frequency analysis of the F2 progenies from selfed heterozygotes revealed a possible pseudo-linkage of sdk1-7 and abi3-6 suggesting a reciprocal translocation event in the heterozygote. Our study emphasizes the importance of robust analysis to distinguish gametophytic defect phenotypes caused by genetic interactions and that resulting from possible chromosomal translocation events.

中文翻译：

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拟南芥 sdk1-7 +/- abi3-6 +/- 杂合子雌配子体缺陷的鉴定和表征

摘要 被子植物的成功繁殖取决于其雄性和雌性配子体的高度同步发育以及随后来自这些生殖组织类型的配子的融合。当穿过 T-DNA 插入系 sdk1-7-/-(Salk_024564) 时，一种参与 ABA 反应的 S 域受体激酶与快速中子缺失系 abi3-6-/-，F1 杂合子 (sdk1-7 +/-abi3-6 +/-) 显示 50% 的胚珠流产，表明可能存在配子体缺陷。我们鉴定并表征了杂合突变胚珠中的早期雌配子体发育缺陷。来自自交杂合子的 F2 后代的重组频率分析显示 sdk1-7 和 abi3-6 可能存在假连锁，表明杂合子中存在相互易位事件。