Abstract

The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo. T. sinensis seeds polysaccharides (TSP) were separated and purified using DEAE-52 cellulose and Sephacryl S-300 column chromatography to obtain TSP-3a characterized of molecular weight, monosaccharides composition, and other preliminary structural features. TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21. FT-IR analysis suggested TSP-3a was an acid polysaccharide and the presence of β configuration within the TSP-3a was discovered. Combining ¹H NMR, ¹³C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.

Recovery of immune activity by administration of polysaccharides of Toona sinensis and its characterization of major componentAll authorsRongshen Wang,Min Zuo,Shihong Ding,Bing Liu,Chao Meng,Bo Song &Wanzhong Lihttps://doi.org/10.1080/14786419.2020.1788558

Published online:

13 July 2020

中文翻译：

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香椿多糖对免疫活性的恢复及其主要成分的表征

摘要

多糖是从种子获取香椿通过热水提取，乙醇沉淀，脱色大孔900树脂，以及脱蛋白与Sevag法用试剂，其具有对IgA，IgG和IgM的水平，所述相对索引显著免疫调节活性胸腺和脾脏，以及小鼠体内的吞噬指数。中华柽柳使用DEAE-52纤维素和Sephacryl S-300柱层析对种子多糖（TSP）进行分离纯化，得到具有分子量、单糖组成和其他初步结构特征的TSP-3a。TSP-3a (758.6 kDa) 主要包含摩尔比为 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21 的 Man、GalN、GlcA、Rha、Glc、Gal、Ara 和 Fuc。FT-IR 分析表明 TSP-3a 是一种酸性多糖，发现 TSP-3a 中存在β构型。组合¹ H NMR，¹³ C NMR，和与那些在文献中报道的对比，TSP-3a的主链可能包含→6） - α -D- Glc-（1→，→3） - β -D- GlcA- (1→, →6)- α -D-Gal-(1→,α -D-Glc-（1→和→6） - β -D-GAL-（1→。

通过多糖的管理免疫活性的恢复香椿，其主要组成部分的表征所有作者荣申王，闵左传·，世红丁，兵刘，诏萌，博宋和万众李https://doi.org/10.1080/14786419.2020 .1788558

在线发布：

2020 年 7 月 13 日