Abstract

Andrographis paniculata is known as the king of bitter and it has been widely used as a medicinal plant. The properties of A. paniculata are generally determined by the metabolite composition, which may be influenced by several factors, one of which is the part of the plant extracted. The objectives of this research are to identify putatively the metabolite composition of the stem and the leaves extracts using LC-MS/MS and classify them using PCA. The stem and the leaves samples were separated and extracted using sonication with 70% ethanol. A total of 31 metabolite compounds has been putatively identified. All compounds were identified in the stem and the leaves extracts, which only differed in their intensity. These metabolite compounds were divided into diterpene lactones, flavonoids, and phenolic acid groups. By using the peak intensities of the 18 compounds identified, the leaves and stem extracts were grouped using PCA.

摘要

穿心莲被称为苦中之王，被广泛用作药用植物。A. paniculata 的特性通常由代谢物组成决定，这可能受到多种因素的影响，其中之一是植物提取的部分。本研究的目的是使用 LC-MS/MS 推定鉴定茎和叶提取物的代谢物组成，并使用 PCA 对其进行分类。使用 70% 乙醇的超声处理分离和提取茎和叶样品。推定鉴定出总共 31 种代谢物化合物。在茎和叶提取物中鉴定出所有化合物，仅在强度上有所不同。这些代谢物化合物分为二萜内酯、类黄酮和酚酸基团。