Abstract

Saussurea lappa (Decne.) Sch.Bip is a well known herbal medicine has been extensively used for many therapeutic purposes. In this article, we have accomplished targeted profiling of S. lappa roots samples collected from different locations (Pangi, Lahaul, Barot and Kullu) of the western Himalayan region using NMR. This study allowed us to assigned four major sesquiterpenes of this plant, including costunolide (CL), dehydrocostus lactone (DCL), alantolactone (AL), isoalantolactone (IAL). Quantification of sesquiterpene lactones have also been accomplished using ¹HNMR, and a wide diversity in the concentration of these active metabolites was observed. It has been observed that AL and IAL were major in the samples collected from Lahaul and Barot regions, and CL and DCL were major in the samples collected from Kullu and Pangi regions. This study concludes that S. lappa roots possess wide chemical diversity, which may be due to various biotic and abiotic environmental related factors.

摘要

Saussurea lappa (Decne.) Sch.Bip 是一种众所周知的草药，已广泛用于许多治疗目的。在本文中，我们使用 NMR 对从喜马拉雅西部地区不同地点（Pangi、Lahaul、Barot 和 Kullu）采集的S. lappa根样本进行了有针对性的分析。这项研究使我们能够分配这种植物的四种主要倍半萜，包括木香内酯 (CL)、脱氢木香内酯 (DCL)、阿内酯 (AL)、异阿内酯 (IAL)。倍半萜内酯的定量也已使用^1完成HNMR，并观察到这些活性代谢物浓度的广泛差异。观察到，在拉哈尔和巴罗地区采集的样本中，AL和IAL以为主，而在库鲁和潘吉地区采集的样本中，CL和DCL为主。本研究得出结论，S. lappa根具有广泛的化学多样性，这可能是由于各种生物和非生物环境相关因素。